This page offers protocols for use with our Hooke Kit™ products. For information on contract research models, please see our CRO page.

Protocols for use with Hooke Kit™ products

Protocol Use with Hooke Kit™ Cat #
Adoptive Transfer EAE in C57BL/6 Mice MOG35-55/CFA Emulsion
MOG35-55 in TC Media, 100x
FBS for T cell culture, 100 mL
FBS for T cell culture, 500 mL
EK-0111
DS-0111
SZ-0101
SZ-0501
Adoptive Transfer EAE in SJL Mice PLP139-151/CFA Emulsion
PLP139-151 in TC Media, 100x
FBS for T cell culture, 100 mL
FBS for T cell culture, 500 mL
EK-0122
DS-0121
SZ-0101
SZ-0501
CIA Induction in DBA/1 Mice Chicken Collagen/CFA Emulsion &
Chicken Collagen/IFA Emulsion
EK-0210 &
EK-0211
Bovine Collagen/CFA Emulsion &
Bovine Collagen/IFA Emulsion
EK-0220 &
EK-0221
Cytokine Production Induced by T Cell Recall Response In Vitro MOG35-55/CFA Emulsion
PLP139-151/CFA Emulsion
MBP1-17/CFA Emulsion
OVA323-339/CFA Emulsion
Antigens in TC media
Fetal bovine serum for T cell culture
EK-0111
EK-0122
EK-0130
EK-0132
(any)
(any)
EAE Induction by Active Immunization in C57BL/6 Mice MOG35-55/CFA Emulsion PTX
MOG1-125/CFA Emulsion PTX
EK-2110
EK-2160
EAE Induction by Active Immunization in Lewis Rats gpMBP69-88/CFA Emulsion
gpMBP69-88/CFA Emulsion PTX
EK-3110
EK-3111
EAE Induction by Active Immunization in SJL Mice PLP139-151/CFA Emulsion
PLP139-151/CFA Emulsion PTX
EK-0120
EK-2120
Immunization of Mice for Generation of Encephalitogenic T Cells MOG35-55/CFA Emulsion
PLP139-151/CFA Emulsion
MBP1-17/CFA Emulsion
EK-0111
EK-0122
EK-0130
Immunization of Mice for Production of Antigen-Specific Antibodies Ovalbumin/CFA Emulsion
Ovalbumin/IFA Emulsion
Pigeon Cytochrome c/CFA Emulsion
Pigeon Cytochrome c/IFA Emulsion
Hen Egg Lysozyme/CFA Emulsion
Hen Egg Lysozyme/IFA Emulsion
EK-0301
EK-0311
EK-0302
EK-0312
EK-0303
EK-0313
Immunization of Mice to Induce DTH Response mBSA/CFA Emulsion EK-0133

Sample IACUC Protocols

These documents are sample IACUC protocols, offered as a convenience to our customers. Your institutional IACUC committee may require changes or additions; these are only a starting point.

Permission is hereby granted to use, modify, and reproduce these protocols without limitation. Credit to Hooke Laboratories is requested as a courtesy, but is not required.

Protocol Sample protocol for use with Hooke Kit™ Cat #
Evaluation of compound effects on EAE development in C57BL/6 mice MOG35-55/CFA Emulsion PTX
MOG1-125/CFA Emulsion PTX
EK-2110
EK-2160