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CIA induction Hooke Kits™

For induction of collagen-induced arthritis (CIA) in DBA/1 mice

Supplied in pre-filled syringes, ready to use.

CIA is induced by chicken or bovine collagen antigen in CFA, followed 18–21 days later by collagen in IFA.

When used with the recommended protocol, about 10–20% of mice develop arthritis within 16 to 20 days, and the remainder within 30 to 35 days of immunization. Most mice reach disease severity of 10 to 14 (on the scale 0 to 16).

Each lot is tested and individually adjusted to ensure consistent disease induction.

Properly prepared emulsions are critical for reliable induction of CIA. Our emulsions are carefully made and pre-filled into syringes, ready to use, to reduce time needed to set up experiments.

Lot-to-lot reagent variations can cause dramatic changes in severity of induced autoimmune disease. The consistent potency of pre-characterized Hooke Kits™ eliminates time-consuming testing.

Pre-filled syringes are prepared under aseptic conditions and delivered in sterilized plastic bags for easy disinfection before introduction into your mouse facility.

Collagen-induced arthritis (CIA) is one of the most commonly used animal models of human rheumatoid arthritis (RA).

Joint inflammation, which develops in animals with CIA, strongly resembles inflammation observed in patients with RA. Blocking tumor necrosis factor (TNF) is efficacious in treatment of CIA, just as it is a highly efficacious therapy in treatment of RA patients.

CIA is mediated by both T-cells and antibodies (B-cells). Macrophages are believed to play an important role in mediating tissue damage during disease development.

CIA is induced by immunization with collagen/CFA emulsion, followed 18–21 days later by collagen/IFA emulsion. See recommended protocol and product details.

While both bovine type II and chicken type II collagen are used for CIA induction, bovine collagen appears to be slightly better at inducing CIA in male DBA/1 mice, while chicken collagen works slightly better in females.

Type II collagen emulsions for CIA induction in DBA/1 mice

Cat # Hooke Kit™ Strain Age Description Size Price
(first kit)
Price (each
add'l kit)
EK-0210 Chicken Collagen/CFA Emulsion DBA/1 7–10 weeks Type II collagen/CFA
emulsion in pre-filled syringe
20 mice $ 362 $ 308
EK-0211 Chicken Collagen/IFA Emulsion DBA/1 7–10 weeks Type II collagen/IFA
emulsion in pre-filled syringe
20 mice $ 339 $ 289
EK-0220 Bovine Collagen/CFA Emulsion DBA/1* 7–10 weeks Type II collagen/CFA
emulsion in pre-filled syringe
20 mice $ 362 $ 308
EK-0221 Bovine Collagen/IFA Emulsion DBA/1* 7–10 weeks Type II collagen/IFA
emulsion in pre-filled syringe
20 mice $ 339 $ 289

* Will also induce CIA in Lewis rats if Hooke's protocol is followed; see here.


These kits can be customized for a small additional charge. Contact us at or with your requirements.

Protocol

CIA Induction in DBA/1 Mice

Detailed contents

Each kit provides sufficient emulsion for 20 mice.

Antigen is chicken type II collagen (EK-0210, EK-0211) or bovine type II collagen (EK-0220, EK-0221).

Chicken Collagen/CFA Emulsion (EK-0210):

Qty Description
1 1 mL syringe, pre-filled with chicken type II collagen emulsion/CFA
~ 1 mg chicken type II collagen/mL emulsion
~ 2 mg killed mycobacterium tuberculosis H37Ra/mL emulsion
1 Data sheet: Recommended experimental protocol, typical results

Chicken Collagen/IFA Emulsion (EK-0211):

Qty Description
1 1 mL syringe, pre-filled with chicken type II collagen emulsion/IFA
~ 1 mg chicken type II collagen/mL emulsion
1 Data sheet: Recommended experimental protocol, typical results

Bovine Collagen/CFA Emulsion (EK-0220):

Qty Description
1 1 mL syringe, pre-filled with bovine type II collagen emulsion/CFA
~ 1 mg bovine type II collagen/mL emulsion
~ 2 mg killed mycobacterium tuberculosis H37Ra/mL emulsion
1 Data sheet: Recommended experimental protocol, typical results

Bovine Collagen/IFA Emulsion (EK-0221):

Qty Description
1 1 mL syringe, pre-filled with bovine type II collagen emulsion/IFA
~ 1 mg bovine type II collagen/mL emulsion
1 Data sheet: Recommended experimental protocol, typical results

Typical results

Results graph

CIA induction in DBA/1 mice

Protocol: CIA Induction in DBA/1 Mice

Data are from three independent experiments using Hooke Kits™ Chicken Collagen/CFA Emulsion (EK-0210) and Chicken Collagen/IFA Emulsion (EK-0211), with female DBA/1 mice (Taconic Biosciences).

Similar results are obtained using Bovine Collagen/CFA Emulsion (EK-0220) and Bovine Collagen/IFA Emulsion (EK-0221) in male DBA/mice.

Exp # Mice/group Age at
immunization
Mean maximum
score ± SD
Day of onset
± SD
Disease
incidence
1 7 7 weeks 12.71 ± 3.98 26.3 ± 6.2 100 %
2 8 8 weeks 12.63 ± 3.24 24.4 ± 3.9 100 %
3 7 8 weeks 13.71 ± 3.72 23.7 ± 5.6 100 %

Frequently asked questions

Is the booster dose (collagen with IFA) really necessary for CIA induction?

Yes, in our experience it is necessary. However, mouse substrains can differ, and a very few labs have reported that their mice develop CIA after a single immunization, without a booster. Most labs need a booster dose for good CIA development. With the booster, disease onset is 5-10 days after the booster in almost all mice. Without the booster, CIA usually develops in only 20-75% of mice, and onset is much more spread over time.

Are there gender differences in CIA development?

We recommend using male mice. CIA develops very similarly in males and females, but females are more likely to develop skin lesions near the injection site; in mice with lesions CIA develops poorly. Males rarely develop skin lesions.

Can these kits be used to induce CIA in Lewis rats?

If Hooke's protocol is followed, our bovine collagen CIA kits will induce CIA in Lewis rats (both males and females).

Use the same protocol recommended for mice, except at double the dose, so 0.1 mL/rat of Bovine Collagen/CFA Emulsion (EK-0220) for the first injection, and then 7 days later, Bovine Collagen/IFA Emulsion (EK-0221) again at 0.1 mL/rat. At this dose, each kit is sufficient for 10 rats.

Storage & stability

Stable for 15 days when stored at 2–4 °C.
Do not freeze.

Product citations

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References

Stuart JM et al, JCI 69:673 (1982)
Holmdahl R et al, Clin Exp Immunol 62:639 (1985)

Material Safety Data Sheets (MSDS)

Chicken Collagen/CFA Emulsion (PDF)
Chicken Collagen/IFA Emulsion (PDF)
Bovine Collagen/CFA Emulsion (PDF)
Bovine Collagen/IFA Emulsion (PDF)

Related products

CK-0210     Hooke Control Kit™ for EK-0210
CK-0211     Hooke Control Kit™ for EK-0211
CK-0220     Hooke Control Kit™ for EK-0220
CK-0221     Hooke Control Kit™ for EK-0221