Product photo

Tissue from mice with CIA

For analysis of gene expression, protein production and biomarkers

Snap-frozen mouse paw, spleen, and serum from DBA/1 mice at well-defined stages and severity of CIA, and control samples. Choose paws from our inventory based on CIA score history, disease stage, gender, or antigen used for CIA induction. Shipped frozen on dry ice.

Save time establishing & running CIA models; most tissue products are available from stock with no lead time.

Full CIA score history provided for each sample.

Control tissues are matched for immunization method and timing.

Availability (IMPORTANT)

Most tissue products are available from stock with no lead time, but contact us at or for a complete listing of our current inventory in Excel format, including CIA score history for each mouse.

You can choose individual left or right hind paws from our stock based on CIA severity, disease stage, gender, or antigen used for CIA induction (chicken type II collagen or bovine type II collagen).

CIA tissue

From DBA/1 mice with CIA.

Cat # Gender Disease stage Tissue Size Price
CZ-0110 Female 24-48 hours after CIA onset Hind paw, spleen, serum Half mouse $ 178
CZ-0111 Male 24-48 hours after CIA onset Hind paw, spleen, serum Half mouse $ 137
CZ-0120 Female 4-5 days after CIA onset Hind paw, spleen, serum Half mouse $ 178
CZ-0121 Male 4-5 days after CIA onset Hind paw, spleen, serum Half mouse $ 137
CZ-0130 Female 2-3 weeks after CIA onset Hind paw, spleen, serum Half mouse $ 178
CZ-0131 Male 2-3 weeks after CIA onset Hind paw, spleen, serum Half mouse $ 137

Control tissue

Control tissue from DBA/1 mice immunized with control kit (no CIA induction), matched for time from immunization.

Cat # Gender Disease stage Tissue Size Price
CZ-0010 Female Immunized only, time matched to 24-48 hours after CIA onset Hind paw, spleen, serum Half mouse $ 226
CZ-0011 Male Immunized only, time matched to 24-48 hours after CIA onset Hind paw, spleen, serum Half mouse $ 185
CZ-0020 Female Immunized only, time matched to 4-5 days after CIA onset Hind paw, spleen, serum Half mouse $ 226
CZ-0021 Male Immunized only, time matched to 4-5 days after CIA onset Hind paw, spleen, serum Half mouse $ 185
CZ-0030 Female Immunized only, time matched to 2-3 weeks after CIA onset Hind paw, spleen, serum Half mouse $ 226
CZ-0031 Male Immunized only, time matched to 2-3 weeks after CIA onset Hind paw, spleen, serum Half mouse $ 185

Naïve tissue

Tissue from naïve DBA/1 mice (no immunization or CIA induction). For control purposes.

Cat # Gender Disease stage Tissue Size Price
CZ-0100 Female Naïve mouse (no immunization or CIA induction) Hind paw, spleen, serum Half mouse $ 76
CZ-0101 Male Naïve mouse (no immunization or CIA induction) Hind paw, spleen, serum Half mouse $ 71

Tissue preparation

Male or female DBA/1 mice 8 to 12 weeks of age are acclimated to our facility for at least 7 days.

CIA is induced according to the protocol CIA Induction in DBA/1 Mice.

Each paw is scored daily for CIA severity on the scale of 0-4 (see protocol) until disease onset, and then every other day. Total score for each mouse is calculated as the sum of scores for all 4 paws (0-16).

Mice are euthanized at the appropriate stage of disease. Immediately, blood is collected for serum, hind paws and spleen are collected and snap frozen in liquid nitrogen. Serum is isolated and frozen at -80 C.

Tissues are stored at -80 C until shipment.

Detailed contents

Qty Description
1 Hind paw, frozen in 1.5 mL cryogenic tube.
Typical CIA paw weight 100-400 mg.
Typical control or naïve paw weight 100-150 mg.
1 Half spleen, frozen in 1.5 mL cryogenic tube.
Typical weight 40-80 mg.
100 µL Serum, frozen in 1.5 mL cryogenic tube.
1 Excel sheet (via email) with CIA score history for all 4 paws.

Each product unit contains tissue and serum from a single mouse. (Spleens and serum are divided and packaged with a single paw because paws of the same mouse can have different arthritis severity.)

Tubes are marked with a mouse ID, paw ID (left or right hind paw), and weight of paw or spleen tissue.

Normally customer orders tissue samples based on CIA score history.

Storage

Keep frozen until use. Storage at -80 C is recommended.

Safety Data Sheet (SDS)

File link (PDF)