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Tissue from mice with CIA

For analysis of gene expression, protein production and biomarkers

Snap frozen mouse paw, spleen, and serum from DBA/1 mice at well-defined stages and severity of CIA. Choose paws from our inventory based on CIA score history, disease stage, gender, or antigen used for CIA induction. Shipped frozen on dry ice.

Save time establishing & running CIA models; most tissue products are available from stock with no lead time.

Full CIA score history provided for each sample.

Control tissues are matched for immunization method and timing.

Availability (IMPORTANT)

Most tissue products are available from stock with no lead time, but contact us at or for a complete listing of our current inventory in a spreadsheet, including CIA score history for each mouse.

You can choose individual left or right hind paws from our stock based on CIA severity, disease stage, gender, or antigen used for CIA induction (chicken type II collagen or bovine type II collagen).

Cat # Strain-Gender Disease stage Tissue Size Price
CZ-0110 DBA/1-F 24-48 hours after CIA onset Hind paw, spleen, serum Half mouse $ 241
CZ-0111 DBA/1-M 24-48 hours after CIA onset Hind paw, spleen, serum Half mouse $ 195
CZ-0120 DBA/1-F 4-5 days after CIA onset Hind paw, spleen, serum Half mouse $ 241
CZ-0121 DBA/1-M 4-5 days after CIA onset Hind paw, spleen, serum Half mouse $ 195
CZ-0130 DBA/1-F 2-3 weeks after CIA onset Hind paw, spleen, serum Half mouse $ 241
CZ-0131 DBA/1-M 2-3 weeks after CIA onset Hind paw, spleen, serum Half mouse $ 195

(See also control and naïve tissue.)

Preparation

Male or female DBA/1 mice 8 to 12 weeks of age are acclimated to our facility for at least 7 days.

CIA is induced according to the protocol CIA Induction in DBA/1 Mice.

Each paw is scored daily for CIA severity on the scale of 0-4 (see protocol) until disease onset, and then every other day. Total score for each mouse is calculated as the sum of scores for all 4 paws (0-16).

Mice are euthanized at the appropriate stage of disease. Immediately, blood is collected for serum, hind paws and spleen are collected and snap frozen in liquid nitrogen. Serum is isolated and frozen at -80 °C.

Tissues are stored at -80 °C until shipment.

Detailed contents

Qty Description
1 Hind paw, frozen in 1.5 mL cryogenic tube.
Typical CIA paw weight 100-400 mg.
Typical control or naïve paw weight 100-150 mg.
1 Half spleen, frozen in 1.5 mL cryogenic tube.
Typical weight 40-80 mg.
100 µL Serum, frozen in 1.5 mL cryogenic tube.
1 Spreadsheet (via email) with CIA score history for all 4 paws.

Each product unit contains tissue and serum from a single mouse. (Spleens and serum are divided and packaged with a single paw because paws of the same mouse can have different arthritis severity.)

Tubes are marked with a mouse ID, paw ID (left or right hind paw), and weight of paw or spleen tissue.

Normally customer orders tissue samples based on CIA score history.

Storage

Keep frozen until use. Storage at -80 °C is recommended.

Safety Data Sheet (SDS)

File link (PDF)

Related products

Serum from mice with CIA
Control and naïve tissue, cells, and serum