Product photo

Tissue from mice with EAE

For analysis of gene expression and protein production

Snap-frozen mouse tissue from well-defined stages and severity of EAE, and control tissues. Choose tissue samples from our inventory based on EAE severity, disease stage, or mouse strain. Shipped frozen on dry ice.

Save time establishing & running EAE models; most tissue products are available from stock with no lead time.

Full EAE score history provided for each sample.

Control tissues are matched for immunization method and timing.

Availability (IMPORTANT)

Most tissue products are available from stock with no lead time, but contact us at or for a complete listing of our current inventory in Excel format, including EAE score history for each mouse.

Choose individual mice from our stock based on EAE severity, disease stage, or mouse strain.

Note: Brain tissue is also available for some mice (add $15/mouse). See our current inventory for availability.

EAE tissue

Cat # Strain Disease stage Tissue Size Price
EZ-1110 C57BL/6 EAE onset Spleen & spinal cord 1 mouse $ 238
EZ-1120 C57BL/6 EAE disease peak Spleen & spinal cord 1 mouse $ 238
EZ-1130 C57BL/6 EAE recovery (chronic phase) Spleen & spinal cord 1 mouse $ 238
EZ-1210 SJL Onset of first wave of EAE Spleen & spinal cord 1 mouse $ 257
EZ-1220 SJL Peak of first wave of EAE Spleen & spinal cord 1 mouse $ 257
EZ-1230 SJL Remission from first wave of EAE Spleen & spinal cord 1 mouse $ 257
EZ-1240 SJL Onset of EAE relapse (within 24 hours after onset) Spleen & spinal cord 1 mouse $ 357
EZ-1250 SJL Peak of EAE relapse (2-3 days after onset) Spleen & spinal cord 1 mouse $ 357
EZ-1260 SJL Recovery from EAE relapse Spleen & spinal cord 1 mouse $ 293

Control tissue

Control tissue from mice immunized with control kit (no EAE induction), matched for time from immunization.

Cat # Strain Disease stage Tissue Size Price
EZ-1111 C57BL/6 Immunized only, time matched to EAE onset Spleen & spinal cord 1 mouse $ 238
EZ-1121 C57BL/6 Immunized only, time matched to EAE peak Spleen & spinal cord 1 mouse $ 238
EZ-1131 C57BL/6 Immunized only, time matched to EAE recovery Spleen & spinal cord 1 mouse $ 238
EZ-1211 SJL Immunized only, time matched to EAE first wave onset Spleen & spinal cord 1 mouse $ 257
EZ-1221 SJL Immunized only, time matched to EAE first wave peak Spleen & spinal cord 1 mouse $ 257
EZ-1231 SJL Immunized only, time matched to EAE first wave remission Spleen & spinal cord 1 mouse $ 257
EZ-1241 SJL Immunized only, time matched to EAE relapse onset Spleen & spinal cord 1 mouse $ 179
EZ-1251 SJL Immunized only, time matched to EAE relapse peak Spleen & spinal cord 1 mouse $ 179
EZ-1261 SJL Immunized only, time matched to EAE relapse recovery Spleen & spinal cord 1 mouse $ 147

Naïve tissue

Tissue from naïve mice (no immunization or EAE induction). For control purposes.

Cat # Strain Disease stage Tissue Size Price
EZ-1100 C57BL/6 Naïve mouse (no immunization or EAE induction) Spleen & spinal cord 1 mouse $ 92
EZ-1200 SJL Naïve mouse (no immunization or EAE induction) Spleen & spinal cord 1 mouse $ 111

Tissue preparation

Female C57BL/6 or SJL mice 8 to 12 weeks of age are acclimated to our facility for at least 7 days.

In C57BL/6 mice, EAE is induced according to the protocol EAE Induction by Active Immunization in C57BL/6 Mice.

In SJL mice, EAE is induced according to the protocol EAE Induction by Active Immunization in SJL Mice.

Mice are scored daily for EAE severity on the scale of 0-5 in 0.5 unit increments (see protocol).

Mice are weighed 3 times/week (Monday, Wednesday, Friday).

Mice are euthanized at the appropriate stage of disease. Mice are immediately perfused with PBS, then spleen & spinal cord are isolated and snap frozen in liquid nitrogen.

Tissues are stored at -80 C until shipment.

Detailed contents

Each product unit contains the entire spleen and spinal cord tissue from a single mouse.

Each organ is supplied in a separate 1.5 mL cryogenic tube. Tubes are marked with a mouse ID and tissue weight. (Typical spinal cords are 80 to 100 mg each, spleens are typically 100 to 200 mg.)

The daily EAE severity scores and weight history for the mouse are provided for each mouse as an Excel sheet, via email. (Normally customer chooses mice for ordering based on EAE score history.)

Storage

Keep frozen until use. Storage at -80 C is recommended.

Safety Data Sheet (SDS)

File link (PDF)