Vial photo

[Ser140]-PLP139-151 in TC Media, 100x

For in vitro restimulation of [Ser140]-PLP139-151-specific T cells

Supplied in vials

Consistently induces in vitro recall responses of T cells isolated from [Ser140]-PLP139-151/CFA-immunized mice

Induces potent encephalitogenic T cells in vitro

[Ser140]-PLP139-151 in TC media is used in vitro to generate encephalitogenic T cells or to evaluate cellular immune responses.

Encephalitogenic cells are generated by culturing splenocytes and lymph node cells from immunized mice for 72 hours in the presence of 20 µg/mL of antigen.

Cat # Product Description Size SDS
(pdf)
Price
DS-0121 [Ser140]-PLP139-151 in TC Media, 100x* Peptide in tissue culture media 2 mg in 1 mL SDS $ 150

Protocols

Adoptive Transfer EAE in SJL Mice
Cytokine Production Induced by T Cell Recall Response In Vitro

Detailed contents

[Ser140]-PLP139-151 (serine substituted myelin proteolipid protein 139-151)
2 mg in 1 mL of tissue culture media (10% fetal bovine serum in RPMI 1640)
Sequence HSLGKWLGHPDKF
Supplied in vials, ready to use

Storage & stability

Stable 3 months (from time of purchase) when stored at -20 °C or one year when stored at -80 °C.
Limit the number of freeze-thaw cycles. Aliquot if using for more than one experiment.

References

Leonard JP et al., Crit Rev Immunol 17:545 (1997)
McRae BL et al., J Neuroimmunol 38:229 (1992)
Tuohy VK et al., J Immunol 142:1523 (1989)
Nicholson LB et al., Proc Natl Acad Sci USA 95:264 (1998)

Safety Data Sheet (SDS)

[Ser140]-PLP139-151 in TC Media, 100x (PDF)

Related products

EK-0120     [Ser140]-PLP139-151/CFA Emulsion
EK-2120     [Ser140]-PLP139-151/CFA Emulsion PTX
EK-0230     PLP139-151 (native)/CFA Emulsion