Ex Vivo T Cell Function Analysis

Spleen and lymph node cells are restimulated with antigen to analyze cytokine production of T cells in vitro. This can be performed either as a separate study, or the end of an EAE study.

Ex Vivo T Cell Function Analysis

Spleens or lymph nodes are collected, and cell suspensions are setup in 96-well plates, with multiple concentrations of the selected antigen in triplicates.

After 72 hours of culture, supernatants are collected and analyzed for cytokines using Luminex, ELISA, or CBA.

Analysis is performed on the supernatant from each well.

Typical results

At the end of a MOG35-55-induced EAE study (29 days post-immunization), spleen and LN cells were restimulated with antigen. Cytokine production was compared between 2 immunized mice that did not develop EAE ("no disease" group) and 6 immunized mice that developed chronic EAE ("chronic" group).

IL-17A - lymph nodes
IL-17A - lymph nodes
IL-17A - spleens
IL-17A - spleens
IFNγ - lymph nodes
IFNγ - lymph nodes
IFNγ - spleens
IFNγ - spleens
TNF - lymph nodes
TNF - lymph nodes
TNF - spleens
TNF - spleens

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