Ex Vivo T Cell Function Analysis
Spleen and lymph node cells are restimulated with antigen to analyze cytokine production of T cells in vitro. Usually this is performed either as a pre-study pilot, or at the end of an study.
Spleens or lymph nodes are collected, and cell suspensions are setup in 96-well plates, with multiple concentrations of the selected antigen in triplicates.
After 72 hours of culture, supernatants are collected and analyzed for cytokines using Luminex, ELISA, or CBA Th1/Th2/Th17 kit (Becton Dickinson).
Analysis is performed on the supernatant from each well (single analysis for each well).
Typical results
At the end of a MOG35-55-induced EAE study (29 days post-immunization), spleen and LN cells were restimulated with antigen. Cytokine production was compared between 2 immunized mice that did not develop EAE ("no disease" group) and 6 immunized mice that developed chronic EAE ("chronic" group).
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