Ex Vivo T Cell Function Analysis
Spleen and lymph node cells are restimulated with antigen to analyze cytokine production of T cells in vitro. This can be performed either as a separate study, or the end of an EAE study.
Spleens or lymph nodes are collected, and cell suspensions are setup in 96-well plates, with multiple concentrations of the selected antigen in triplicates.
After 72 hours of culture, supernatants are collected and analyzed for cytokines using Luminex, ELISA, or CBA.
Analysis is performed on the supernatant from each well.
Typical results
At the end of a MOG35-55-induced EAE study (29 days post-immunization), spleen and LN cells were restimulated with antigen. Cytokine production was compared between 2 immunized mice that did not develop EAE ("no disease" group) and 6 immunized mice that developed chronic EAE ("chronic" group).
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