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Tissue from mice with SLE

For analysis of gene expression, protein production and biomarkers

Snap frozen tissue from mouse strains that spontaneously develop disease similar to systemic lupus erythematosus (SLE).

Save time aging mice as disease develops; tissue is available from stock with no lead time.

Tissue from young mice (prior to proteinuria development) - negative control for disease
Tissue from cyclophosphamide-treated mice - positive control for treatment

Tissue from (NZB x NZW)F1 mice

From female (NZB x NZW)F1 hybrid (NZBWF1) mice at 15 to 52 weeks of age.

These crossbred mice spontaneously develop a polygenic autoimmune disease closely resembling systemic lupus erythematosus (SLE) in human patients. Anti-nuclear and anti-dsDNA antibodies are detectable as early as 20 weeks of age. Proteinuria and kidney pathology (immune complex glomerulonephritis) are observed at 36 to 45 weeks.

Cat # Mouse strain Treatment/proteinuria score Price
LZ-0100 (NZB x NZW)F1 hybrid (NZBWF1) Cyclophosphamide-treated (no or mild proteinuria; < 30 mg protein/dL) $ 568
LZ-0101 (NZB x NZW)F1 hybrid (NZBWF1) Vehicle-treated or naïve (severe proteinuria; > 500 mg protein/dL) $ 650
LZ-0102 (NZB x NZW)F1 hybrid (NZBWF1) Naïve, 15 to 18 weeks old (no or mild proteinuria; < 30 mg protein/dL) $ 307

Tissue from MRL/lpr mice

From female MRL/lpr mice at 6 to 22 weeks of age.

MRL/lpr mice are homozygous for spontaneous mutation in the Fas gene, and develop lymphadenopathy (enlarged lymph nodes), splenomegaly (enlarged spleen) and systemic autoimmunity.

Autoimmune responses occur spontaneously in these mice, resulting in arthritis and glomerulonephritis resembling SLE in people. Anti-nuclear and anti-dsDNA antibodies are detectable as early as 8 weeks of age. Proteinuria and kidney pathology (immune complex glomerulonephritis) are found at 16 to 28 weeks of age.

Cat # Mouse strain Treatment/proteinuria score Price
LZ-0200 MRL/lpr Cyclophosphamide-treated (no or mild proteinuria; < 30 mg protein/dL) $ 449
LZ-0201 MRL/lpr Vehicle-treated or naïve (severe proteinuria; > 500 mg protein/dL) $ 624
LZ-0202 MRL/lpr Naïve, 6 to 8 weeks old (no or mild proteinuria; < 30 mg protein/dL) $ 339

Preparation

Blood is immediately collected for serum, organs are collected and snap frozen in liquid nitrogen. Serum is isolated and frozen at -80 °C.

All tissue is stored at -80 °C until shipment.

Detailed contents

Qty Description
1 Kidney, frozen in marked 1.5 mL cryogenic tube.
1 Half spleen, frozen in marked 1.5 mL cryogenic tube.
150 µL Whole blood, frozen in marked 1.5 mL cryogenic tube.
150 µL Serum, frozen in marked 1.5 mL cryogenic tube.

Tubes are marked with mouse ID, treatment, age at termination, tissue type, and tissue weight or volume.

Each product unit contains tissue and serum from a single mouse.

Storage

Keep frozen until use. Storage at -80 °C is recommended.

Safety Data Sheet (SDS)

File link (PDF)