Delayed-Type Hypersensitivity (DTH)

DTH is a short-term model for evaluation of compound effects on cellular immune response, primarily Th1 and Th17 type responses.

Female C57BL/6 mice are immunized with methylated bovine serum albumin (mBSA) emulsified in complete Freund's adjuvant (CFA).

Five days later, mice are challenged in one hind foot pad with soluble mBSA. The other hind foot pad is injected with PBS and serves as a negative control. Differences in paw swelling and weight between the mBSA and PBS-injected paws are the primary readouts, measured 24 hours after the challenge.

Myeloperoxidase (MPO) activity and cytokine concentrations (IL-17A, IL-6, TNF and IL-10) can also be measured in homogenized paw tissue.

Test compounds can be dosed from the time of immunization (equivalent to prophylactic dosing), or immediately before the challenge (equivalent to therapeutic dosing).

Positive control is dexamethasone, but we have also seen efficacy with FTY720.

This model is useful as a low-cost, short-term (6 day) screen prior to running inflammation models such as CIA or EAE.

This model can also be run in other mouse strains.

Please contact Hooke at or with questions or for a quotation.

Typical results

DTH paw thickness results DTH paw weight results DTH MPO results

Click here (XLS file) to download the raw data & analysis from this study (this is not a full study report).

References

[1] Kim YS, Maslinski W, Zheng XX, Stevens AC, Li XC, Tesch GH, Kelley VR, Strom TB, J Immunol. 160:5742-8 (1998)
[2] Särnstrand B, Jansson AH, Matuseviciene G, Scheynius A, Pierrou S, Bergstrand H, J Pharmacol Exp Ther. 288:1174-84 (1999)
[3] Yoshimoto T, Wang CR, Yoneto T, Matsuzawa A, Cruikshank WW, Nariuchi H, Blood 95:2869-74 (2000)