Spinal Cord Homogenate Induced EAE in (BALB/c x SJL)F1 Mice
DISCONTINUED MODEL
Effective February 2023, Hooke no longer offers this model. While it has been published as an efficacy test for glatiramer acetate (generic Copaxone), our experience (with concurrence from the FDA) is that other models are better suited for that purpose.
This page remains online primarily to prevent old links to it from breaking, and for reference purposes.
Hooke runs most commonly-used EAE models. Click here for an overview of EAE and a list of models offered.
This direct EAE model is induced by immunization with mouse whole spinal cord homogenate emulsified in CFA in crossbred (BALB/c x SJL)F1 mice, followed by a single injection of pertussis toxin.
It was once commonly used to evaluate efficacy and equivalence of generic glatiramer acetate [1], however our experience (with concurrence from the FDA) is that other models are better suited for that purpose.
Copaxone (Teva Pharmaceuticals) is used as a positive control and reference compound. Treatment is prophylactic, the model is 28 days long.
Glatiramer acetate potency can alternatively be evaluated by analysis of cytokine production by T cells isolated from immunized mice; Hooke offers ELISA, cytokine bead assay (CBA), and Luminex assays, which are all suitable for this analysis.
Please contact Hooke at or with questions or for a quotation.
Typical results in this model
Spinal cord homogenate induced EAE in (BALB/c x SJL)F1 mice
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| Treatment | Median day of onset |
p value (onset) |
MMS ± SEM |
p value (MMS) |
Relapse incidence | p value (incidence) |
|---|---|---|---|---|---|---|
| Vehicle | 10.0 | 3.50 ± 0.19 | 81.8% | |||
| Glatiramer acetate | 13.0 | <0.0001 | 1.83 ± 0.33 | 0.0015 | 33.3% | 0.0162 |
| Treatment | Relapse MMS ± SEM |
p value (relapse MMS) |
End score ± SEM |
p value (end score) |
End weight (%) ± SEM |
p value (end weight) |
|---|---|---|---|---|---|---|
| Vehicle | 2.83 ± 0.32 | 1.92 ± 0.43 | 91.6 ± 1.8 | |||
| Glatiramer acetate | 0.96 ± 0.36 | 0.0015 | 0.79 ± 0.34 | 0.0172 | 103.8 ± 2.3 | 0.0004 |
Tissue collection and end-of-study analysis
Hooke offers an extensive set of tissue collection and analysis options. Click here for more information.
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