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Fresh cells from immunized mice

For in vitro analysis of T cells, encephalitogenic cells, or culture for adoptive transfer EAE

Fresh splenocytes and/or lymph node cells from mice immunized with antigen.

Fresh cell suspensions shipped cold overnight, ready for use.

Consistent immunization and cell isolation protocols yield consistent cell quality.

Cells from CFA-only immunized mice and naïve mice available for comparison.

Lead time (IMPORTANT)

Orders must be placed 3 weeks before shipment. Each cell product order is custom prepared.

Preparation

Female mice of age 10 to 14 weeks are acclimated to our facility for at least 7 days, then immunized with antigen subcutaneously in the lower back, close to the base of tail, as follows:

Strain Hooke Kit™ used for
antigen immunization
Hooke Kit™ used for
CFA-only immunization
Hooke Kit™ used
for naïve mice
C57BL/6 EK-0111 (MOG35-55) CK-0111 (CFA emulsion) none
SJL EK-0122 (PLP139-151) CK-0122 (CFA emulsion) none

11 days later (10 days for SJL mice), mice are euthanized and cell suspension(s) from spleen and/or inguinal lymph nodes are prepared.

Red blood cells in spleen are lysed.

Cells are resuspended in complete tissue culture media and cell concentrations are determined and marked on tubes.

Cells are shipped the same day for overnight delivery.

Cells from antigen immunized mice

Cat # Strain Antigen Product Size Price
(first mouse)
Price (each
add'l mouse)
CF-1001 C57BL/6 MOG35-55 Spleen cells 1 mouse $ 141 $ 111
CF-1002 C57BL/6 MOG35-55 Lymph node cells 1 mouse $ 141 $ 111
CF-1003 C57BL/6 MOG35-55 Spleen cells and lymph node cells 1 mouse $ 184 $ 154
CF-2001 SJL PLP139-151 Spleen cells 1 mouse $ 160 $ 130
CF-2002 SJL PLP139-151 Lymph node cells 1 mouse $ 160 $ 130
CF-2003 SJL PLP139-151 Spleen cells and lymph node cells 1 mouse $ 203 $ 173

Cells from CFA-only immunized (control) mice

Cat # Strain Antigen Product Size Price
(first mouse)
Price (each
add'l mouse)
CF-1101 C57BL/6 CFA only Spleen cells 1 mouse $ 141 $ 111
CF-1102 C57BL/6 CFA only Lymph node cells 1 mouse $ 141 $ 111
CF-1103 C57BL/6 CFA only Spleen cells and lymph node cells 1 mouse $ 184 $ 154
CF-2101 SJL CFA only Spleen cells 1 mouse $ 160 $ 130
CF-2102 SJL CFA only Lymph node cells 1 mouse $ 160 $ 130
CF-2103 SJL CFA only Spleen cells and lymph node cells 1 mouse $ 203 $ 173

Cells from non-immunized (naïve) mice

Cat # Strain Antigen Product Size Price
(first mouse)
Price (each
add'l mouse)
CF-1201 C57BL/6 none Spleen cells 1 mouse $ 119 $ 89
CF-1202 C57BL/6 none Lymph node cells 1 mouse $ 119 $ 89
CF-1203 C57BL/6 none Spleen cells and lymph node cells 1 mouse $ 162 $ 132
CF-2201 SJL none Spleen cells 1 mouse $ 138 $ 108
CF-2202 SJL none Lymph node cells 1 mouse $ 138 $ 108
CF-2203 SJL none Spleen cells and lymph node cells 1 mouse $ 181 $ 151

Controls

For some assays (i.e. protein and gene expression analysis) spleen or lymph node cells from naïve mice or mice immunized with CFA-only should be used as negative controls.

Detailed contents

Centrifuge tubes (15 or 50 mL) with cells in complete tissue culture media (90% RPMI 1640, 10% fetal calf serum, supplemented with 0.1 mM MEM non-essential amino acids, 1 mM MEM sodium pyruvate, 2 mM L-glutamine, 100 UI/mL penicillin, 100 µg/mL streptomycin, 5 x 10-5 M 2-mercaptoethanol).

Each cell type is supplied in a separate centrifuge tube, together with cell counts (cells/mL) for the cell type. If cells from multiple mice (of the same catalog number) are ordered, each type of cells will be pooled in one tube with one cell count.

(Please specify if you want separate tubes for each mouse instead of pooled cells; in that case the "first mouse" price applies.)

Each product unit contains the entire suspension of spleen and/or lymph node cells from one mouse.

Cell type Cells/mouse
(immunized)
Cells/mouse
(naïve)
Splenocytes 100-200 x 106 50-100 x 106
Lymph node cells 10-30 x 106 1-3 x 106

Storage & stability

Fresh cells ship cold on gel packs in insulated packaging. They should be used or frozen within 24 hours of shipment.

For this reason this product ships to US and Canadian addresses only, via FedEx overnight.

(We can supply frozen cells to international customers at an additional charge of $25/item. These ship on dry ice. Please specify "frozen cells" when ordering.)

Safety Data Sheet (SDS)

Fresh cells from immunized mice (PDF)

Related products

DS-0111     MOG35-55 in TC Media, 100x
DS-0121     PLP139-151 in TC Media, 100x