Cells in vial

Control and Naive Tissue, Cells, and Serum

For analysis of gene expression and protein production

Snap frozen tissue, fresh cells, and frozen serum from naïve mice (no immunization) and control mice (immunized with adjuvant only).

These products are for use as negative controls, for comparison to disease-induced mice. Tissue is time-matched to tissue from diseased mice.

Save time establishing & running disease models.

Contact or for pricing and details.

Availability and lead time (IMPORTANT)

Most frozen products are available from stock with no lead time, but contact us at or for current inventory.

Fresh cell orders are custom prepared and require lead time - please contact us.

Fresh cell products are also available as frozen cell aliquots at an additional charge of $25 per vial (minimum charge $200/order). Those ship on dry ice. Please specify "frozen cells" when ordering.

Note: Brain tissue is also available for some mice (add $24/mouse). See our current inventory for availability.

CIA control tissue and serum

Snap frozen tissue from mice immunized with adjuvant only (control kit; no CIA induction), matched for time from CIA induction.

Cat # Strain-Gender Termination Tissue Size Price
CZ-0010 DBA/1-F Time matched to 24-48 hrs after CIA onset Hind paw, spleen, serum Half mouse $ 300
CZ-0011 DBA/1-M Time matched to 24-48 hrs after CIA onset Hind paw, spleen, serum Half mouse $ 300
CZ-0020 DBA/1-F Time matched to 4-5 days after CIA onset Hind paw, spleen, serum Half mouse $ 300
CZ-0021 DBA/1-M Time matched to 4-5 days after CIA onset Hind paw, spleen, serum Half mouse $ 300
CZ-0030 DBA/1-F Time matched to 2-3 weeks after CIA onset Hind paw, spleen, serum Half mouse $ 300
CZ-0031 DBA/1-M Time matched to 2-3 weeks after CIA onset Hind paw, spleen, serum Half mouse $ 300

(See also CIA tissue.)

Frozen serum from mice immunized with adjuvant only (control kit).

Cat # Strain Termination Tissue Size Price
CZ-0270 DBA/1 35 to 60 days after immunization Serum 1 mL $ 828
CZ-5270 DBA/1 35 to 60 days after immunization Serum 250 µL $ 357

(See also CIA serum.)

EAE control tissue and cells

Snap frozen tissue from mice immunized with adjuvant only (control kit; no EAE induction), matched for time from EAE induction.

Cat # Strain Termination Tissue Size Price
EZ-1111 C57BL/6 Time matched to EAE onset Spleen & spinal cord 1 mouse $ 307
EZ-1121 C57BL/6 Time matched to EAE peak Spleen & spinal cord 1 mouse $ 307
EZ-1131 C57BL/6 Time matched to EAE recovery Spleen & spinal cord 1 mouse $ 307
EZ-1211 SJL Time matched to EAE first wave onset Spleen & spinal cord 1 mouse $ 334
EZ-1221 SJL Time matched to EAE first wave peak Spleen & spinal cord 1 mouse $ 334
EZ-1231 SJL Time matched to EAE first wave remission Spleen & spinal cord 1 mouse $ 334
EZ-1241 SJL Time matched to EAE relapse onset Spleen & spinal cord 1 mouse $ 334
EZ-1251 SJL Time matched to EAE relapse peak Spleen & spinal cord 1 mouse $ 334
EZ-1261 SJL Time matched to EAE relapse recovery Spleen & spinal cord 1 mouse $ 334

(See also EAE tissue.)

Fresh cells from mice immunized with adjuvant only (control kit; no EAE induction). Termination at age 8 to 18 weeks.

Cat # Strain Antigen Product Size Price
(first mouse)
Price (each
add'l mouse)
CF-1101 C57BL/6 CFA only Spleen cells 1 mouse $ 382 $ 232
CF-1102 C57BL/6 CFA only Lymph node cells 1 mouse $ 382 $ 232
CF-1103 C57BL/6 CFA only Spleen cells and lymph node cells 1 mouse $ 448 $ 298
CF-2101 SJL CFA only Spleen cells 1 mouse $ 409 $ 259
CF-2102 SJL CFA only Lymph node cells 1 mouse $ 409 $ 259
CF-2103 SJL CFA only Spleen cells and lymph node cells 1 mouse $ 475 $ 325

Naïve tissue, cells, and serum

Snap frozen tissue from naïve mice (no immunization or disease induction).

Cat # Strain-Gender Termination Tissue Size Price
CZ-0100 DBA/1-F Age 8 to 18 weeks Hind paw, spleen, serum Half mouse $ 114
CZ-0101 DBA/1-M Age 8 to 18 weeks Hind paw, spleen, serum Half mouse $ 108
EZ-1100 C57BL/6 Age 8 to 18 weeks Spleen & spinal cord 1 mouse $ 129
EZ-1200 SJL Age 8 to 18 weeks Spleen & spinal cord 1 mouse $ 156

Fresh cells from naïve mice (no immunization or disease induction). Termination at age 8 to 18 weeks.

Cat # Strain Antigen Product Size Price
(first mouse)
Price (each
add'l mouse)
CF-1201 C57BL/6 none Spleen cells 1 mouse $ 321 $ 171
CF-1202 C57BL/6 none Lymph node cells 1 mouse $ 321 $ 171
CF-1203 C57BL/6 none Spleen cells and lymph node cells 1 mouse $ 387 $ 237
CF-2201 SJL none Spleen cells 1 mouse $ 348 $ 198
CF-2202 SJL none Lymph node cells 1 mouse $ 348 $ 198
CF-2203 SJL none Spleen cells and lymph node cells 1 mouse $ 414 $ 264

Frozen serum from naïve mice (no immunization or disease induction).

Cat # Strain Termination Tissue Size Price
CZ-0280 DBA/1 Age 8 to 18 weeks Serum 1 mL $ 491
CZ-5280 DBA/1 Age 8 to 18 weeks Serum 250 µL $ 180
SZ-0281 C57BL/6 Age 8 to 18 weeks Serum 1 mL $ 478
SZ-5281 C57BL/6 Age 8 to 18 weeks Serum 250 µL $ 175
SZ-0283 BALB/c Age 8 to 18 weeks Serum 1 mL $ 464
SZ-5283 BALB/c Age 8 to 18 weeks Serum 250 µL $ 170

Preparation

Mice 8 to 14 weeks of age are acclimated to our facility for at least 7 days.

For products from immunized mice (does not apply to products from naïve mice), mice are then immunized with adjuvant(s) only as follows:

Mouse strain Hooke Control Kit(s)™ used for immunization Using protocol
DBA/1 (CIA controls) CK-0210 & CK-0211 (CFA & IFA emulsions) CIA Induction in DBA/1 Mice
C57BL/6 (EAE controls) CK-2110 (CFA emulsion) EAE Induction by Active Immunization in C57BL/6 Mice
SJL (EAE controls) CK-0120 (CFA emulsion) EAE Induction by Active Immunization in SJL Mice

Mice are euthanized at the timepoints given above.

Snap frozen tissue products

If serum is included, blood is collected immediately after termination and serum is isolated.

All tissue is snap frozen in liquid nitrogen and stored at -80 °C until shipment.

Fresh cell products

Cell suspensions are prepared and red blood cells in spleen are lysed.

Cells are resuspended in complete tissue culture media and cell concentrations are determined and marked on tubes.

Cells are shipped the same day for overnight delivery.

Frozen serum products

At termination blood is immediately collected, and serum is isolated.

Serum from multiple mice is pooled and stored at -80 °C until shipment.

Detailed contents - snap frozen tissue

Each product unit contains tissue and/or serum from a single mouse.

Each organ is supplied in a separate 1.5 mL cryogenic tube. Tubes are marked with a mouse ID, paw ID (left or right hind paw; if applicable), and tissue weight.

Typical tissue quantity:

Detailed contents - fresh cells

Each product unit contains the entire suspension of spleen and/or lymph node cells from one mouse.

Cells are supplied in 15 or 50 mL centrifuge tubes, in complete tissue culture media (90% RPMI 1640, 10% fetal calf serum, supplemented with 0.1 mM MEM non-essential amino acids, 1 mM MEM sodium pyruvate, 2 mM L-glutamine, 100 UI/mL penicillin, 100 µg/mL streptomycin, 5 x 10-5 M 2-mercaptoethanol).

Each cell type is supplied in a separate centrifuge tube, together with cell counts (cells/mL) for the cell type. If cells from multiple mice (of the same catalog number) are ordered, each type of cell will be pooled in one tube with one cell count.

(Please specify if you want separate tubes for each mouse instead of pooled cells; in that case the "first mouse" price applies.)

The typical number of cells expected for naïve and immunized mice is as follows:

Cell type Cells/mouse
(immunized)
Cells/mouse
(naïve)
Splenocytes 100 to 200 x 106 50 to 100 x 106
Lymph node cells 10 to 30 x 106 1 to 3 x 106

Detailed contents - frozen serum

Frozen serum, pooled from multiple mice, supplied in a 1.5 mL cryogenic tube on dry ice.

Storage & stability

Frozen tissue and serum ship on dry ice. Keep frozen until use. Storage at -80 °C is recommended.

Fresh cells ship cold on gel packs in insulated packaging. Use or freeze within 24 hours of shipment. For this reason fresh cells ship to US and Canadian addresses only, via FedEx overnight. (These products can be shipped as frozen cell aliquots to other locations, as described above.)

Safety Data Sheet (SDS)

Frozen tissue and serum SDS (PDF)
Fresh cell products SDS (PDF)

Related products

Tissue from mice with CIA
Serum from mice with CIA
Tissue from mice with EAE
Control and naïve tissue, cells, and serum