gpMBP69-88/CFA Emulsion for EAE Induction in Lewis Rats
By active immunization
Emulsion supplied in pre-filled syringes, ready to use.
- Consistently induces EAE in female Lewis rats
EAE will develop in 90 to 100% of rats within 10 to 14 days of immunization. Maximum score for individual rats will typically be 3.0 to 3.5.
Each lot is tested and individually adjusted to ensure consistent disease induction.
- Eliminates tedious preparation of emulsions
Properly prepared emulsions are critical for reliable induction of many autoimmune disease models. Our emulsions are carefully made and pre-filled into syringes, ready to use, to reduce time needed to set up experiments.
- Saves time and rats in testing individual reagents
Lot-to-lot reagent variations can cause dramatic changes in severity of induced autoimmune disease. The consistent potency of pre-characterized Hooke Kits™ eliminates time-consuming testing.
- Reduces your rodent facility's exposure to pathogen contamination
Pre-filled syringes are prepared under aseptic conditions and delivered in sterilized plastic bags for easy disinfection before introduction into your rodent facility.
Experimental autoimmune encephalomyelitis (EAE) is the model most commonly used to study efficacy of potential drugs for treatment of multiple sclerosis (MS).
The EAE model in Lewis rats has consistent disease onset and severity, as well as a short clinical course, making it a popular compound screen for potential efficacy in MS.
EAE in Lewis rats is characterized by paralysis and CNS inflammation. Demyelination is not typically observed. EAE is initiated by myelin-specific CD4+ T cells, with glia cells (microglia and astrocytes), B cells, macrophages, NK cells, dendritic cells, and mast cells all likely playing roles in disease development.
Most investigators immunize Lewis rats with gpMBP69-88 to induce EAE (cat. no. EK-3110, without pertussis toxin). In this model rats fully recover by Day 20 after immunization.
It is possible to induce more severe EAE by administering pertussis toxin (PTX) to rats one day after immunization. If PTX is administered in addition to the antigen, 30% to 60% of rats will experience a relapse within 28 days from immunization. This provides an opportunity for study of mechanism of EAE development and relapses.
For more information on model selection, please see Learning Center - What are the advantages and disadvantages of the different EAE models and antigens?.
| Cat # | Hooke Kit™ | Strain | Age | Description | Size | Price (first kit) |
Price (each add'l kit) |
|---|---|---|---|---|---|---|---|
| EK-3110 | gpMBP69-88/CFA Emulsion | Lewis rats | 9 to 14 weeks | Emulsion in syringes | 10 rats | $ 358 | $ 304 |
Hooke kits™ can be customized for a small additional charge. Contact us at or with your requirements.
Protocol
EAE Induction by Active Immunization in Lewis Rats
Detailed contents
Each kit provides sufficient reagents for 10 rats.
Antigen is myelin basic protein 69-88, guinea pig (gpMBP69-88), sequence YGSLPQKSQRSQDENPVVHF.
| Qty | Description |
|---|---|
| 3 | Syringes pre-filled with 0.7 mL gpMBP69-88/CFA emulsion ~ 0.5 mg gpMBP69-88/mL emulsion ~ 2-5 mg killed mycobacterium tuberculosis H37Ra/mL emulsion (all concentrations adjusted by lot for consistent EAE induction) |
| 1 | Data sheet: Recommended experimental protocol, typical results |
Typical results
Protocol: EAE Induction by Active Immunization in Lewis Rats
Note: EK-3111 has been discontinued, but similar results will be obtained by immunizing rats with EK-3110 and injecting PTX (such as Hooke BT-0105) one day after immunization.
| Number of rats | Age at immunization | Mean maximum score ± SD |
Mean day of onset ± SD |
Disease incidence |
|---|---|---|---|---|
| 7 | 12 weeks | 3.1 ± 0.6 | 11.4 ± 0.8 | 100% |
Storage & stability
Stable for 20 days when stored at 2–4 °C.
Do not freeze.
References
[1] McFarlin DE, Blank SE, Kibler RF, J. Immunol. 1 13:712 (1974)
[2] Kardys E and Hashim GA, J Immunol 127:862 (1981)
[3] Mannie MD, Paterson PY, U'Prichard DC, Flouret G., Proc Natl Acad Sci USA 82:5515 (1985)
[4] Hashim GA, Day ED, Fredane L, Intintola P, Carvalho E, J Neurosci Res. 16(3):467-78 (1986)
Safety Data Sheets (SDS)
gpMBP69-88/CFA Emulsion (PDF)
Related products
CK-3110
Hooke Control Kit™ for EK-3110 (full size)
CK-8110
Hooke Control Kit™ for EK-3110 (half size)
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