MOG1-125/CFA Emulsion PTX 3.75X
For EAE induction by active immunization in C57BL/6 mice
DISCONTINUED PRODUCT
Replaced by Hooke Kit™ MOG1-125/CFA Emulsion PTX
(cat. no. EK-2160), effective October 2012.
This product has been discontinued and replaced. This page is for historical reference only.
All customers are encouraged to switch to the latest products for this application.
There are no advantages to the discontinued product.
Product selection | Protocol | Contents | Typical results | Storage | SDS | Related products
Supplied in pre-filled syringes, ready to use.
- Consistently induces EAE in female C57BL/6 mice
EAE will develop in 90-100% of mice within 8 to 14 days, with mean maximum score of 3.0 to 3.5. Each lot is tested and individually adjusted to ensure consistent disease induction.
On Day 0, MOG1-125/CFA emulsion and pertussis
toxin are injected.
On Day 1, a second dose of pertussis toxin is injected.
- Eliminates tedious preparation of emulsions
Properly prepared emulsions are critical for reliable induction of many autoimmune disease models. Our emulsions are carefully made and pre-filled into syringes, ready to use, to reduce time needed to set up experiments.
- Saves time and mice in testing individual reagents
Lot-to-lot reagent variations can cause dramatic changes in severity of induced autoimmune disease. The consistent potency of pre-characterized Hooke Kits™ eliminates time-consuming testing.
- Reduces your mouse facility's exposure to pathogen contamination
Pre-filled syringes are prepared under aseptic conditions and delivered in sterilized plastic bags for easy disinfection before introduction into your mouse facility.
| Cat # | Hooke Kit™ | Strain | PTX dose | Description | Size | Price (first kit) |
Price (each add'l kit) |
|---|---|---|---|---|---|---|---|
| EK-0162 | MOG1-125/CFA Emulsion PTX 2.5X | C57BL/6 | 2.5x | Emulsion, PTX in pre-filled syringes |
10 mice | DISCONTINUED | |
| EK-0164 | MOG1-125/CFA Emulsion PTX 3.75X | C57BL/6 | 3.75x | Emulsion, PTX in pre-filled syringes |
10 mice | DISCONTINUED | |
These kits can be customized for a small additional charge. Contact us at or with your requirements.
Product selection (IMPORTANT)
Many factors – including mouse age, strain, breeder, and colony – influence EAE disease susceptibility and the pertussis toxin (PTX) dose required. Even with identical mice and reagents, EAE disease can vary considerably from lab to lab.
Due to the high cost of reagents in these products, we do not offer free samples of MOG1-125 Hooke Emulsion Kits™.
Instead, we recommend that customers titrate EAE development using our MOG35-55/CFA Emulsion PTX Hooke Kits™ and then select a MOG1-125 Hooke Kit™ based on the results as follows:
| Best EAE development with MOG35-55 |
Recommended MOG1-125 Hooke Kit™ |
|---|---|
| EK-0110 or EK-0112 | EK-0162 |
| EK-0114 or EK-0115 | EK-0164 |
Please contact us for information about sample ordering for this purpose.
With a given Hooke Kit™, older mice generally develop more severe disease and more uniform disease onset.
Protocol
EAE Induction by Active Immunization in C57BL/6 Mice
Detailed contents
Each kit provides sufficient reagents for 10 mice.
Antigen is human recombinant myelin oligodendrocyte glycoprotein (1-125) (MOG1-125).
| Qty | Description |
|---|---|
| 2 | 1 mL syringes, pre-filled with human recombinant MOG1-125/CFA emulsion
~ 0.5 mg MOG1-125/mL emulsion ~ 2 mg killed mycobacterium tuberculosis H37Ra/mL emulsion |
| 2 | 1 mL syringes, pre-filled with pertussis toxin in phosphate
buffered saline (PBS) ~ 1–6 µg pertussis toxin/mL of PBS |
| 1 | Data sheet: Recommended experimental protocol, typical results |
Typical results
EAE induction in C57BL/6 mice
Protocol: EAE Induction by Active Immunization in C57BL/6 Mice
Data are from three independent experiments using Hooke Kit™ MOG35-55/CFA Emulsion PTX (EK-0110), with Taconic mice.
Similar results are obtained using other MOG35-55/CFA Emulsion PTX Hooke Kits™, and with MOG1-125/CFA Emulsion PTX Hooke Kits™, using the recommended protocol.
| Exp # | Mice/group | Age at immunization |
Mean maximum score ± SD |
Day of onset ± SD |
Disease incidence |
|---|---|---|---|---|---|
| 1 | 10 | 10 weeks | 3.25 ± 0.49 | 10.9 ± 3.9 | 100 % |
| 2 | 10 | 10 weeks | 3.45 ± 0.60 | 10.1 ± 1.5 | 100 % |
| 3 | 8 | 9 weeks | 3.25 ± 0.38 | 12.1 ± 1.6 | 100 % |
Storage & stability
Stable for 20 days when stored at 2–4 °C.
Do not freeze.
Safety Data Sheets (SDS)
MOG1-125/CFA Emulsion (PDF)
Pertussis Toxin in PBS (PDF)
Related products
CK-0162 Hooke Control Kit™ for EK-0162 (full size)
CK-0164 Hooke Control Kit™ for EK-0164 (full size)
CK-5162 Hooke Control Kit™ for EK-0162 (half size)
CK-5164 Hooke Control Kit™ for EK-0164 (half size)
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