Product photo

MOG35-55/CFA Emulsion PTX 2.5X

For EAE induction by active immunization in C57BL/6 mice

DISCONTINUED PRODUCT

Replaced by Hooke Kit™ MOG35-55/CFA Emulsion PTX
(cat. no. EK-2110), effective October 2012.

This product has been discontinued and replaced. This page is for historical reference only.

All customers are encouraged to switch to the latest products for this application.
There are no advantages to the discontinued product.

Product selection | Protocol | Contents | Typical Results | Storage | SDS | Related products

Supplied in pre-filled syringes, ready to use.

EAE will develop in 90-100% of mice within 8 to 14 days, with mean maximum score of 3.0 to 3.5. Each lot is tested and individually adjusted to ensure consistent disease induction.

On Day 0, MOG35-55/CFA emulsion and pertussis toxin are injected.
On Day 1, a second dose of pertussis toxin is injected.

Properly prepared emulsions are critical for reliable induction of many autoimmune disease models. Our emulsions are carefully made and pre-filled into syringes, ready to use, to reduce time needed to set up experiments.

Lot-to-lot reagent variations can cause dramatic changes in severity of induced autoimmune disease. The consistent potency of pre-characterized Hooke Kits™ eliminates time-consuming testing.

Pre-filled syringes are prepared under aseptic conditions and delivered in sterilized plastic bags for easy disinfection before introduction into your mouse facility.

Cat # Hooke Kit™ Strain PTX dose Description Size Price
(first kit)		 Price (each
add'l kit)
EK-0113 MOG35-55/CFA Emulsion PTX 1X C57BL/6 1x Emulsion, PTX in
pre-filled syringes		 10 mice DISCONTINUED
EK-0110 MOG35-55/CFA Emulsion PTX 1.5X C57BL/6 1.5x Emulsion, PTX in
pre-filled syringes		 10 mice DISCONTINUED
EK-0112 MOG35-55/CFA Emulsion PTX 2.5X C57BL/6 2.5x Emulsion, PTX in
pre-filled syringes		 10 mice DISCONTINUED
EK-0114 MOG35-55/CFA Emulsion PTX 3.75X C57BL/6 3.75x Emulsion, PTX in
pre-filled syringes		 10 mice DISCONTINUED
EK-0115 MOG35-55/CFA Emulsion PTX 5X C57BL/6 5x Emulsion, PTX in
pre-filled syringes		 10 mice DISCONTINUED
EK-0116 MOG35-55/CFA Emulsion PTX 7X C57BL/6 7x Emulsion, PTX in
pre-filled syringes		 10 mice DISCONTINUED

These kits can be customized for a small additional charge. Contact us at or with your requirements.

Product selection (IMPORTANT)

Many factors – including mouse age, strain, breeder, and colony – influence EAE disease susceptibility and the pertussis toxin (PTX) dose required. Even with identical mice and reagents, EAE disease can vary considerably from lab to lab. 

Therefore, before ordering for the first time, we recommend that you run 10 mice with Hooke Kit™ (EK-0114) and let us recommend the best Hooke Kit™ for you based on the results (EAE incidence and average severity). After running 10 mice with EK-0114 you can move to a weaker or stronger kit if necessary, depending on results. Please contact us for information about sample ordering for this purpose.

With a given Hooke Kit™, older mice generally develop more severe disease and more uniform disease onset.

Protocol

EAE Induction by Active Immunization in C57BL/6 Mice

Detailed contents

Each kit provides sufficient reagents for 10 mice.

Antigen is myelin oligodendrocyte glycoprotein 35-55 (MOG35-55) rat, mouse, sequence MEVGWYRSPFSRVVHLYRNGK.

Qty Description
2 1 mL syringes, pre-filled with MOG35-55/CFA emulsion
~ 1 mg MOG35-55/mL emulsion
~ 2 mg killed mycobacterium tuberculosis H37Ra/mL emulsion
2 1 mL syringes, pre-filled with pertussis toxin in phosphate buffered saline (PBS)
~ 0.7–10 µg pertussis toxin/mL of PBS
1 Data sheet: Recommended experimental protocol, typical results

Typical results

Results graph

EAE induction in C57BL/6 mice

Protocol: EAE Induction by Active Immunization in C57BL/6 Mice

Data are from three independent experiments using Hooke Kit™ MOG35-55/CFA Emulsion PTX (EK-0110), with Taconic mice.

Similar results are obtained using other MOG35-55/CFA Emulsion PTX Hooke Kits™, and with MOG1-125/CFA Emulsion PTX Hooke Kits™, using the recommended protocol.

Exp # Mice/group Age at
immunization		 Mean maximum
score ± SD		 Day of onset
± SD		 Disease
incidence
1 10 10 weeks 3.25 ± 0.49 10.9 ± 3.9 100 %
2 10 10 weeks 3.45 ± 0.60 10.1 ± 1.5 100 %
3 8 9 weeks 3.25 ± 0.38 12.1 ± 1.6 100 %

Storage & stability

Stable for 20 days when stored at 2–4 °C.
Do not freeze.

Safety Data Sheets (SDS)

MOG35-55/CFA Emulsion (PDF)

Pertussis Toxin in PBS (PDF)

Related products

CK-0110     Hooke Control Kit™ for EK-0110 (full size)
CK-0112     Hooke Control Kit™ for EK-0112 (full size)
CK-0113     Hooke Control Kit™ for EK-0113 (full size)
CK-0114     Hooke Control Kit™ for EK-0114 (full size)
CK-0115     Hooke Control Kit™ for EK-0115 (full size)
CK-0116     Hooke Control Kit™ for EK-0116 (full size)

CK-5110     Hooke Control Kit™ for EK-0110 (half size)
CK-5112     Hooke Control Kit™ for EK-0112 (half size)
CK-5113     Hooke Control Kit™ for EK-0113 (half size)
CK-5114     Hooke Control Kit™ for EK-0114 (half size)
CK-5115     Hooke Control Kit™ for EK-0115 (half size)
CK-5116     Hooke Control Kit™ for EK-0116 (half size)