Tissue From Mice With SLE
For analysis of gene expression, protein production and biomarkers
Snap frozen tissue from mouse strains that spontaneously develop disease similar to systemic lupus erythematosus (SLE).
- Analyze gene expression, protein presence, and biomarkers in tissue without a vivarium
Save time aging mice as disease develops; tissue is available from stock with no lead time.
- Control tissues available for comparison
Tissue from young mice (prior to proteinuria development) - negative control for disease
Tissue from cyclophosphamide-treated mice - positive control for treatment
Tissue from (NZB x NZW)F1 mice
From female (NZB x NZW)F1 hybrid (NZBWF1) mice at 15 to 52 weeks of age.
These crossbred mice spontaneously develop a polygenic autoimmune disease closely resembling systemic lupus erythematosus (SLE) in human patients. Anti-nuclear and anti-dsDNA antibodies are detectable as early as 20 weeks of age. Proteinuria and kidney pathology (immune complex glomerulonephritis) are observed at 36 to 45 weeks.
| Cat # | Mouse strain | Treatment/proteinuria score | Price |
|---|---|---|---|
| LZ-0100 | (NZB x NZW)F1 hybrid (NZBWF1) | Cyclophosphamide-treated (no or mild proteinuria; < 30 mg protein/dL) | $ 625 |
| LZ-0101 | (NZB x NZW)F1 hybrid (NZBWF1) | Vehicle-treated or naïve (severe proteinuria; > 500 mg protein/dL) | $ 715 |
| LZ-0102 | (NZB x NZW)F1 hybrid (NZBWF1) | Naïve, 15 to 18 weeks old (no or mild proteinuria; < 30 mg protein/dL) | $ 338 |
Tissue from MRL/lpr mice
From female MRL/lpr mice at 6 to 22 weeks of age.
MRL/lpr mice are homozygous for spontaneous mutation in the Fas gene, and develop lymphadenopathy (enlarged lymph nodes), splenomegaly (enlarged spleen) and systemic autoimmunity.
Autoimmune responses occur spontaneously in these mice, resulting in arthritis and glomerulonephritis resembling SLE in human patients. Anti-nuclear and anti-dsDNA antibodies are detectable as early as 8 weeks of age. Proteinuria and kidney pathology (immune complex glomerulonephritis) are found at 16 to 28 weeks of age.
| Cat # | Mouse strain | Treatment/proteinuria score | Price |
|---|---|---|---|
| LZ-0200 | MRL/lpr | Cyclophosphamide-treated (no or mild proteinuria; < 30 mg protein/dL) | $ 494 |
| LZ-0201 | MRL/lpr | Vehicle-treated or naïve (severe proteinuria; > 500 mg protein/dL) | $ 686 |
| LZ-0202 | MRL/lpr | Naïve, 6 to 8 weeks old (no or mild proteinuria; < 30 mg protein/dL) | $ 373 |
Preparation
Blood is immediately collected for serum, organs are collected and snap frozen in liquid nitrogen. Serum is isolated and frozen at -80 °C.
All tissue is stored at -80 °C until shipment.
Detailed contents
| Qty | Description |
|---|---|
| 1 | Kidney, frozen in marked 1.5 mL cryogenic tube. |
| 1 | Half spleen, frozen in marked 1.5 mL cryogenic tube. |
| 150 µL | Whole blood, frozen in marked 1.5 mL cryogenic tube. |
| 150 µL | Serum, frozen in marked 1.5 mL cryogenic tube. |
Tubes are marked with mouse ID, treatment, age at termination, tissue type, and tissue weight or volume.
Each product unit contains tissue and serum from a single mouse.
Storage
Keep frozen until use. Storage at -80 °C is recommended.
Safety Data Sheet (SDS)
File link (PDF)
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