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Serum From Mice With CIA

For isolation or analysis of anti-collagen antibodies

Preparation | Contents | Storage | SDS (pdf) Related products

Frozen serum from DBA/1 mice with CIA, and serum from control and naïve mice. Shipped frozen on dry ice.

Isolate anti-collagen antibodies from serum of mice with CIA

Source of high titer anti-chicken type II collagen or anti-bovine type II collagen mouse antibodies.

Analyze CIA serum without a vivarium

Save time establishing & running CIA models; serum products normally available from stock with no lead time.

Serum from control and naïve mice available for comparison

Control serum is matched for immunization method and timing.

Serum will be positive for anti-collagen antibodies in ELISA assays at dilutions of 1:100,000 or more.

Serum may be used to isolate anti-collagen antibodies or as a standard for measurement of anti-collagen antibodies in ELISA assays. To generate the standard curve, we recommend starting at 1:1000 dilution and making 1:3 or 1:5 serial dilutions.

For our own studies at Hooke, we normally test one lot of serum and then freeze many 25 µL aliquots for use as standards in multiple ELISA assays.

Cat #	Strain	Antigen	Disease stage	Tissue	Size	Price
CZ-0242	DBA/1	Chicken collagen	20+ days after CIA onset	Serum	1 mL	$ 735
CZ-5242	DBA/1	Chicken collagen	20+ days after CIA onset	Serum	250 µL	$ 305
CZ-0243	DBA/1	Bovine collagen	20+ days after CIA onset	Serum	1 mL	$ 735
CZ-5243	DBA/1	Bovine collagen	20+ days after CIA onset	Serum	250 µL	$ 305

(See also control and naïve tissue.)

Preparation

DBA/1 mice 8 to 12 weeks of age are acclimated to our facility for at least 7 days.

CIA is induced according to the protocol CIA Induction in DBA/1 Mice.

Mice are euthanized after 20 or more days of clinical CIA (typically at 35 to 50 days after immunization). Blood is immediately collected and serum is isolated and frozen at -80 °C.

Serum from multiple mice is pooled and stored at -80 °C until shipment.