Control and Naive Tissue, Cells, and Serum
For analysis of gene expression and protein production
Snap frozen tissue, fresh cells, and frozen serum from naïve mice (no immunization) and control mice (immunized with adjuvant only).
These products are for use as negative controls, for comparison to disease-induced mice. Tissue is time-matched to tissue from diseased mice.
- Analyze gene expression, protein presence, and biomarkers without a vivarium
Save time establishing & running disease models.
- All products can be customized to your requirements
Contact or for pricing and details.
Availability and lead time (IMPORTANT)
Most frozen products are available from stock with no lead time, but contact us at or for current inventory.
Fresh cell orders are custom prepared and require lead time - please contact us.
Fresh cell products are also available as frozen cell aliquots at an additional charge of $25 per vial (minimum charge $200/order). Those ship on dry ice. Please specify "frozen cells" when ordering.
Note: Brain tissue is also available for some mice (add $24/mouse). See our current inventory for availability.
CIA control tissue and serum
Snap frozen tissue from mice immunized with adjuvant only (control kit; no CIA induction), matched for time from CIA induction.
| Cat # | Strain-Gender | Termination | Tissue | Size | Price |
|---|---|---|---|---|---|
| CZ-0010 | DBA/1-F | Time matched to 24-48 hrs after CIA onset | Hind paw, spleen, serum | Half mouse | $ 300 |
| CZ-0011 | DBA/1-M | Time matched to 24-48 hrs after CIA onset | Hind paw, spleen, serum | Half mouse | $ 300 |
| CZ-0020 | DBA/1-F | Time matched to 4-5 days after CIA onset | Hind paw, spleen, serum | Half mouse | $ 300 |
| CZ-0021 | DBA/1-M | Time matched to 4-5 days after CIA onset | Hind paw, spleen, serum | Half mouse | $ 300 |
| CZ-0030 | DBA/1-F | Time matched to 2-3 weeks after CIA onset | Hind paw, spleen, serum | Half mouse | $ 300 |
| CZ-0031 | DBA/1-M | Time matched to 2-3 weeks after CIA onset | Hind paw, spleen, serum | Half mouse | $ 300 |
(See also CIA tissue.)
Frozen serum from mice immunized with adjuvant only (control kit).
| Cat # | Strain | Termination | Tissue | Size | Price |
|---|---|---|---|---|---|
| CZ-0270 | DBA/1 | 35 to 60 days after immunization | Serum | 1 mL | $ 828 |
| CZ-5270 | DBA/1 | 35 to 60 days after immunization | Serum | 250 µL | $ 357 |
(See also CIA serum.)
EAE control tissue and cells
Snap frozen tissue from mice immunized with adjuvant only (control kit; no EAE induction), matched for time from EAE induction.
| Cat # | Strain | Termination | Tissue | Size | Price |
|---|---|---|---|---|---|
| EZ-1111 | C57BL/6 | Time matched to EAE onset | Spleen & spinal cord | 1 mouse | $ 307 |
| EZ-1121 | C57BL/6 | Time matched to EAE peak | Spleen & spinal cord | 1 mouse | $ 307 |
| EZ-1131 | C57BL/6 | Time matched to EAE recovery | Spleen & spinal cord | 1 mouse | $ 307 |
| EZ-1211 | SJL | Time matched to EAE first wave onset | Spleen & spinal cord | 1 mouse | $ 334 |
| EZ-1221 | SJL | Time matched to EAE first wave peak | Spleen & spinal cord | 1 mouse | $ 334 |
| EZ-1231 | SJL | Time matched to EAE first wave remission | Spleen & spinal cord | 1 mouse | $ 334 |
| EZ-1241 | SJL | Time matched to EAE relapse onset | Spleen & spinal cord | 1 mouse | $ 334 |
| EZ-1251 | SJL | Time matched to EAE relapse peak | Spleen & spinal cord | 1 mouse | $ 334 |
| EZ-1261 | SJL | Time matched to EAE relapse recovery | Spleen & spinal cord | 1 mouse | $ 334 |
(See also EAE tissue.)
Fresh cells from mice immunized with adjuvant only (control kit; no EAE induction). Termination at age 8 to 18 weeks.
| Cat # | Strain | Antigen | Product | Size | Price (first mouse) |
Price (each add'l mouse) |
|---|---|---|---|---|---|---|
| CF-1101 | C57BL/6 | CFA only | Spleen cells | 1 mouse | $ 382 | $ 232 |
| CF-1102 | C57BL/6 | CFA only | Lymph node cells | 1 mouse | $ 382 | $ 232 |
| CF-1103 | C57BL/6 | CFA only | Spleen cells and lymph node cells | 1 mouse | $ 448 | $ 298 |
| CF-2101 | SJL | CFA only | Spleen cells | 1 mouse | $ 409 | $ 259 |
| CF-2102 | SJL | CFA only | Lymph node cells | 1 mouse | $ 409 | $ 259 |
| CF-2103 | SJL | CFA only | Spleen cells and lymph node cells | 1 mouse | $ 475 | $ 325 |
Naïve tissue, cells, and serum
Snap frozen tissue from naïve mice (no immunization or disease induction).
| Cat # | Strain-Gender | Termination | Tissue | Size | Price |
|---|---|---|---|---|---|
| CZ-0100 | DBA/1-F | Age 8 to 18 weeks | Hind paw, spleen, serum | Half mouse | $ 114 |
| CZ-0101 | DBA/1-M | Age 8 to 18 weeks | Hind paw, spleen, serum | Half mouse | $ 108 |
| EZ-1100 | C57BL/6 | Age 8 to 18 weeks | Spleen & spinal cord | 1 mouse | $ 129 |
| EZ-1200 | SJL | Age 8 to 18 weeks | Spleen & spinal cord | 1 mouse | $ 156 |
Fresh cells from naïve mice (no immunization or disease induction). Termination at age 8 to 18 weeks.
| Cat # | Strain | Antigen | Product | Size | Price (first mouse) |
Price (each add'l mouse) |
|---|---|---|---|---|---|---|
| CF-1201 | C57BL/6 | none | Spleen cells | 1 mouse | $ 321 | $ 171 |
| CF-1202 | C57BL/6 | none | Lymph node cells | 1 mouse | $ 321 | $ 171 |
| CF-1203 | C57BL/6 | none | Spleen cells and lymph node cells | 1 mouse | $ 387 | $ 237 |
| CF-2201 | SJL | none | Spleen cells | 1 mouse | $ 348 | $ 198 |
| CF-2202 | SJL | none | Lymph node cells | 1 mouse | $ 348 | $ 198 |
| CF-2203 | SJL | none | Spleen cells and lymph node cells | 1 mouse | $ 414 | $ 264 |
Frozen serum from naïve mice (no immunization or disease induction).
| Cat # | Strain | Termination | Tissue | Size | Price |
|---|---|---|---|---|---|
| CZ-0280 | DBA/1 | Age 8 to 18 weeks | Serum | 1 mL | $ 491 |
| CZ-5280 | DBA/1 | Age 8 to 18 weeks | Serum | 250 µL | $ 180 |
| SZ-0281 | C57BL/6 | Age 8 to 18 weeks | Serum | 1 mL | $ 478 |
| SZ-5281 | C57BL/6 | Age 8 to 18 weeks | Serum | 250 µL | $ 175 |
| SZ-0283 | BALB/c | Age 8 to 18 weeks | Serum | 1 mL | $ 464 |
| SZ-5283 | BALB/c | Age 8 to 18 weeks | Serum | 250 µL | $ 170 |
Preparation
Mice 8 to 14 weeks of age are acclimated to our facility for at least 7 days.
For products from immunized mice (does not apply to products from naïve mice), mice are then immunized with adjuvant(s) only as follows:
| Mouse strain | Hooke Control Kit(s)™ used for immunization | Using protocol |
|---|---|---|
| DBA/1 (CIA controls) | CK-0210 & CK-0211 (CFA & IFA emulsions) | CIA Induction in DBA/1 Mice |
| C57BL/6 (EAE controls) | CK-2110 (CFA emulsion) | EAE Induction by Active Immunization in C57BL/6 Mice |
| SJL (EAE controls) | CK-0120 (CFA emulsion) | EAE Induction by Active Immunization in SJL Mice |
Mice are euthanized at the timepoints given above.
Snap frozen tissue products
If serum is included, blood is collected immediately after termination and serum is isolated.
All tissue is snap frozen in liquid nitrogen and stored at -80 °C until shipment.
Fresh cell products
Cell suspensions are prepared and red blood cells in spleen are lysed.
Cells are resuspended in complete tissue culture media and cell concentrations are determined and marked on tubes.
Cells are shipped the same day for overnight delivery.
Frozen serum products
At termination blood is immediately collected, and serum is isolated.
Serum from multiple mice is pooled and stored at -80 °C until shipment.
Detailed contents - snap frozen tissue
Each product unit contains tissue and/or serum from a single mouse.
Each organ is supplied in a separate 1.5 mL cryogenic tube. Tubes are marked with a mouse ID, paw ID (left or right hind paw; if applicable), and tissue weight.
Typical tissue quantity:
- Spinal cord 80 to 100 mg
- Spleen 100 to 200 mg
- Half spleen 40 to 80 mg
- Hind paw 100 to 150 mg
- Serum 100 µL
Detailed contents - fresh cells
Each product unit contains the entire suspension of spleen and/or lymph node cells from one mouse.
Cells are supplied in 15 or 50 mL centrifuge tubes, in complete tissue culture media (90% RPMI 1640, 10% fetal calf serum, supplemented with 0.1 mM MEM non-essential amino acids, 1 mM MEM sodium pyruvate, 2 mM L-glutamine, 100 UI/mL penicillin, 100 µg/mL streptomycin, 5 x 10-5 M 2-mercaptoethanol).
Each cell type is supplied in a separate centrifuge tube, together with cell counts (cells/mL) for the cell type. If cells from multiple mice (of the same catalog number) are ordered, each type of cell will be pooled in one tube with one cell count.
(Please specify if you want separate tubes for each mouse instead of pooled cells; in that case the "first mouse" price applies.)
The typical number of cells expected for naïve and immunized mice is as follows:
| Cell type | Cells/mouse (immunized) |
Cells/mouse (naïve) |
|---|---|---|
| Splenocytes | 100 to 200 x 106 | 50 to 100 x 106 |
| Lymph node cells | 10 to 30 x 106 | 1 to 3 x 106 |
Detailed contents - frozen serum
Frozen serum, pooled from multiple mice, supplied in a 1.5 mL cryogenic tube on dry ice.
Storage & stability
Frozen tissue and serum ship on dry ice. Keep frozen until use. Storage at -80 °C is recommended.
Fresh cells ship cold on gel packs in insulated packaging. Use or freeze within 24 hours of shipment. For this reason fresh cells ship to US and Canadian addresses only, via FedEx overnight. (These products can be shipped as frozen cell aliquots to other locations, as described above.)
Safety Data Sheet (SDS)
Frozen tissue and serum SDS (PDF)
Fresh cell products SDS (PDF)
Related products
Tissue from mice with CIA
Serum from mice with CIA
Tissue from mice with EAE
Control and naïve tissue, cells, and serum
_150px.jpg)