CIA Induction Kits
For induction of collagen-induced arthritis (CIA) in DBA/1 mice
Supplied in pre-filled syringes, ready to use.
CIA is induced by chicken or bovine type II collagen in CFA, followed by a type II collagen booster 18 to 21 days later. The collagen booster is usually administered in IFA. Alternatively, the booster can be administered in CFA, resulting in faster CIA development and increased severity.
- Consistently induces arthritis in 90 to 100% of DBA/1 mice (Taconic DBA1BO)
When used with the recommended protocol, 90 to 100% of mice develop arthritis within 35 days of immunization. Most mice reach disease severity of 10 to 14 (on the scale 0 to 16).
Each lot is tested and individually adjusted to ensure consistent disease induction.
- Eliminate tedious preparation of emulsions
Properly prepared emulsions are critical for reliable induction of CIA. Our emulsions are carefully made and pre-filled into syringes, ready to use, to reduce time needed to set up experiments.
- Save time and mice in testing individual reagents
Lot-to-lot reagent variations can cause dramatic changes in severity of induced autoimmune disease. The consistent potency of pre-characterized Hooke Kits™ eliminates time-consuming testing.
- Reduce your mouse facility's exposure to pathogen contamination
Pre-filled syringes are prepared under aseptic conditions and delivered in sterilized plastic bags for easy disinfection before introduction into your mouse facility.
Collagen-induced arthritis (CIA) is one of the most commonly used animal models of human rheumatoid arthritis (RA).
Joint inflammation, which develops in animals with CIA, strongly resembles inflammation observed in patients with RA. Blocking tumor necrosis factor (TNF) is efficacious in treatment of CIA, just as it is a highly efficacious therapy in treatment of RA patients.
CIA is mediated by both T cells and antibodies (B cells). Macrophages are believed to play an important role in mediating tissue damage during disease development.
CIA is induced by immunization with type II collagen/CFA emulsion, followed 18 to 21 days later by collagen/IFA or collagen/CFA emulsion. See recommended protocol.
While both bovine type II and chicken type II collagen are used for CIA induction, bovine collagen appears to be slightly better at inducing CIA in male DBA/1 mice, while chicken collagen works slightly better in females.
Type II collagen emulsions for CIA induction in DBA/1 mice
| Cat # | Hooke Kit™ | Strain1 | Age | Description | Size | Price (first kit) |
Price (each add'l kit) |
|---|---|---|---|---|---|---|---|
| EK-0210 | Chicken Collagen/CFA Emulsion | DBA/1 | 7 to 9 weeks | Type II collagen/CFA emulsion in syringe |
20 mice | $ 427 | $ 363 |
| EK-0211 | Chicken Collagen/IFA Emulsion | DBA/1 | 7 to 9 weeks | Type II collagen/IFA emulsion in syringe |
20 mice | $ 400 | $ 340 |
| EK-0220 | Bovine Collagen/CFA Emulsion | DBA/12 | 7 to 9 weeks | Type II collagen/CFA emulsion in syringe |
20 mice | $ 427 | $ 363 |
| EK-0221 | Bovine Collagen/IFA Emulsion | DBA/12 | 7 to 9 weeks | Type II collagen/IFA emulsion in syringe |
20 mice | $ 400 | $ 340 |
1 Breeder substrain can substantially affect CIA development. Hooke recommends use of Taconic DBA1BO mice with these kits.
2 Will also induce CIA in Lewis rats if Hooke's protocol is followed; see here.
These kits can be customized for a small additional charge. Contact us at or with your requirements.
Protocol
Detailed contents
Each kit provides sufficient emulsion for 20 mice.
Antigen is chicken type II collagen (EK-0210, EK-0211) or bovine type II collagen (EK-0220, EK-0221).
Chicken Collagen/CFA Emulsion (EK-0210):
| Qty | Description |
|---|---|
| 2 |
Syringes pre-filled with 0.5 mL chicken type II collagen emulsion/CFA ~ 1 mg chicken type II collagen/mL emulsion ~ 1 mg killed mycobacterium tuberculosis H37Ra/mL emulsion |
| 1 | Data sheet: Recommended experimental protocol, typical results |
Chicken Collagen/IFA Emulsion (EK-0211):
| Qty | Description |
|---|---|
| 2 |
Syringes pre-filled with 0.5 mL chicken type II collagen emulsion/IFA ~ 1 mg chicken type II collagen/mL emulsion |
| 1 | Data sheet: Recommended experimental protocol, typical results |
Bovine Collagen/CFA Emulsion (EK-0220):
| Qty | Description |
|---|---|
| 2 |
Syringes pre-filled with 0.5 mL bovine type II collagen emulsion/CFA ~ 1 mg bovine type II collagen/mL emulsion ~ 1 mg killed mycobacterium tuberculosis H37Ra/mL emulsion |
| 1 | Data sheet: Recommended experimental protocol, typical results |
Bovine Collagen/IFA Emulsion (EK-0221):
| Qty | Description |
|---|---|
| 2 |
Syringes pre-filled with 0.5 mL bovine type II collagen emulsion/IFA ~ 1 mg bovine type II collagen/mL emulsion |
| 1 | Data sheet: Recommended experimental protocol, typical results |
Typical results
CIA induction in DBA/1 mice – collagen/CFA, collagen/IFA booster
| Exp # | # mice | Age at immunization |
Mean maximum score ± SD |
Day of onset ± SD |
Disease incidence |
|---|---|---|---|---|---|
| 1 | 7 | 7 weeks | 12.71 ± 3.98 | 26.3 ± 6.2 | 100 % |
| 2 | 8 | 8 weeks | 12.63 ± 3.24 | 24.4 ± 3.9 | 100 % |
| 3 | 7 | 8 weeks | 13.71 ± 3.72 | 23.7 ± 5.6 | 100 % |
Protocol: CIA Induction in DBA/1 Mice
Data are from three independent experiments using Hooke Kits™ Chicken Collagen/CFA Emulsion (EK-0210) and Chicken Collagen/IFA Emulsion (EK-0211), with female DBA/1 mice (Taconic Biosciences).
Similar results are obtained using Bovine Collagen/CFA Emulsion (EK-0220) and Bovine Collagen/IFA Emulsion (EK-0221) in male DBA/mice (Taconic Biosciences).
For stronger CIA with earlier onset, use a booster dose containing collagen/CFA (instead of collagen/IFA). The graph below presents a comparison between CFA and IFA-containing boosters:
CIA induction in DBA/1 mice - CFA vs. IFA booster
| Group | # mice | Age at immunization |
Mean maximum score ± SD |
Day of onset ± SD |
Disease incidence |
|---|---|---|---|---|---|
| CFA booster | 10 | 9 weeks | 13.20 ± 3.79 | 23.6 ± 1.5 | 100 % |
| IFA booster | 9 | 9 weeks | 10.33 ± 1.09 | 25.2 ± 2.8 | 100 % |
Data obtained using Hooke Kits™ Bovine Collagen/CFA Emulsion (EK-0220) and Bovine Collagen/IFA Emulsion (EK-0221) in male DBA/mice (Taconic Biosciences).
Frequently asked questions
Is the source of DBA/1 mice important?
CIA development is sensitive to DBA/1 mouse substrain. Hooke recommends use of Taconic Biosciences DBA1BO mice with these kits. Our testing shows arthritis will be consistently induced in 90 to 100% of DBA/1 mice from Taconic. Results may differ in mice from other breeders.
Is the booster dose (collagen with IFA) really necessary for CIA induction?
Yes, in our experience it is necessary. However, mouse substrains can differ, and a very few labs have reported that their mice develop CIA after a single immunization, without a booster. Most labs need a booster dose for good CIA development. With the booster, disease onset is 5-10 days after the booster in almost all mice. Without the booster, CIA usually develops in only 20-75% of mice, and onset is much more spread over time.
Are there gender differences in CIA development?
We recommend using male mice. CIA develops very similarly in males and females, but females are more likely to develop skin lesions near the injection site; in mice with lesions CIA develops poorly. Males rarely develop skin lesions.
Can these kits be used to induce CIA in Lewis rats?
If Hooke's protocol is followed, our bovine collagen CIA kits will induce CIA in Lewis rats (both males and females).
Use the same protocol recommended for mice, except at double the dose, so 0.1 mL/rat of Bovine Collagen/CFA Emulsion (EK-0220) for the first injection, and then 7 days later, Bovine Collagen/IFA Emulsion (EK-0221) again at 0.1 mL/rat. At this dose, each kit is sufficient for 10 rats.
Storage & stability
Stable for 15 days when stored at 2–4 °C.
Do not freeze.
Product citations
Search Google Scholar for product citations (opens in new tab)
References
Stuart JM et al, JCI 69:673 (1982)
Holmdahl R et al, Clin Exp Immunol 62:639 (1985)
Safety Data Sheets (SDS)
Chicken Collagen/CFA Emulsion (PDF)
Chicken Collagen/IFA Emulsion (PDF)
Bovine Collagen/CFA Emulsion (PDF)
Bovine Collagen/IFA Emulsion (PDF)
Related products
CK-0210 Hooke Control Kit™ for EK-0210
CK-0211 Hooke Control Kit™ for EK-0211
CK-0220 Hooke Control Kit™ for EK-0220
CK-0221 Hooke Control Kit™ for EK-0221
Tissue from mice with CIA
Serum from mice with CIA
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