Product photo

[Ser140]-PLP139-151/CFA Emulsion PTX

For EAE induction by active immunization in SJL mice

DISCONTINUED PRODUCT

Replaced by Hooke Kit™ [Ser140]-PLP139-151/CFA Emulsion PTX
(cat. no. EK-2120), effective October 2012.

This product has been discontinued and replaced. This page is for historical reference only.

All customers are encouraged to switch to the latest products for this application.
There are no advantages to the discontinued product.

Protocol | Contents | Storage | SDS | Related Products

Supplied in pre-filled syringes, ready to use.

Each lot is tested and individually adjusted to ensure consistent disease induction.

Properly prepared emulsions are critical for reliable induction of many autoimmune disease models. Our emulsions are carefully made and pre-filled into syringes, ready to use, to reduce time needed to set up experiments.

Lot-to-lot reagent variations can cause dramatic changes in severity of induced autoimmune disease. The consistent potency of pre-characterized Hooke Kits™ eliminates time-consuming testing.

Pre-filled syringes are prepared under aseptic conditions and delivered in sterilized plastic bags for easy disinfection before introduction into your mouse facility.

Cat # Hooke Kit™ Strain PTX
syringes		 Description SDS (pdf) Size Price
(first kit)		 Price (each
add'l kit)
EK-0121 [Ser140]-PLP139-151/CFA Emulsion PTX SJL 1 Emulsion, PTX in pre-filled syringes EmulsionPTX 10 mice DISCONTINUED
EK-0123 [Ser140]-PLP139-151/CFA Emulsion PTXx2 SJL 2 Emulsion, PTX in pre-filled syringes EmulsionPTX 10 mice DISCONTINUED

Protocol

EAE Induction by Active Immunization in SJL Mice

Detailed contents

Each kit provides sufficient reagents for 10 mice.

Antigen is myelin proteolipid protein 139-151 ([Ser140]-PLP139-151), sequence HSLGKWLGHPDKF.

Qty Description
2 1 mL syringes, pre-filled with [Ser140]-PLP139-151/CFA emulsion
~ 1 mg [Ser140]-PLP139-151/mL emulsion
~ 2 mg killed mycobacterium tuberculosis H37Ra/mL emulsion
1 (EK-0121)
2 (EK-0123)		 1 mL syringes, pre-filled with pertussis toxin in phosphate buffered saline (PBS)
2 ± 1 µg pertussis toxin/mL of PBS
1 Data sheet: Recommended experimental protocol, typical results

Storage & stability

Stable for 20 days when stored at 2–4 °C.
Do not freeze.

Safety Data Sheets (SDS)

[Ser140]-PLP139-151/CFA Emulsion (emulsion syringe only) (PDF)

[Ser140]-PLP139-151/CFA Emulsion PTX (PTX syringe only) (PDF)

Related products

CK-0120     Hooke Control Kit™ for EK-0120 (full size)
CK-0121     Hooke Control Kit™ for EK-0121 (full size)
CK-0123     Hooke Control Kit™ for EK-0123 (full size)

CK-5120     Hooke Control Kit™ for EK-0120 (half size)
CK-5121     Hooke Control Kit™ for EK-0121 (half size)
CK-5123     Hooke Control Kit™ for EK-0123 (half size)