Vial photo

MBP1-17 in TC Media, 100x

For in vitro restimulation of MBP1-17-specific T cells

Supplied in vials

Consistently induces in vitro recall responses of T cells isolated from MBP1-17/CFA-immunized mice

Induces potent encephalitogenic T cells in vitro

MBP1-17 in TC media is used in vitro to generate encephalitogenic T cells or to evaluate cellular immune responses.

Encephalitogenic cells are generated by culturing splenocytes from immunized mice for 72 hours in the presence of 20 µg/mL of antigen. To generate encephalitogenic cells, IL-23 or IL-12 and anti-IFNγ antibodies may be added to the culture.

Cat # Product Description Size SDS
(pdf)
Price
DS-0131 MBP1-17 in TC Media, 100x Peptide in tissue culture media 2 mg in 1 mL SDS $ 150

Protocol

Cytokine Production Induced by T Cell Recall Response In Vitro

Detailed contents

Myelin basic protein 1-17 (MBP1-17)
2 mg in 1 mL of tissue culture media (10% fetal bovine serum in RPMI 1640)
Mouse sequence ASQKRPSQRSKYLATAS
Supplied in vials, ready to use

Storage & stability

Stable 3 months (from time of purchase) when stored at -20 °C or one year when stored at -80 °C.
Limit the number of freeze-thaw cycles. Aliquot if using for more than one experiment.

References

Perrin PJ et al, J Immunol 163;1704 (1999)
Sabelko-Downes KA et al, J Exp Med 189:1195 (1999)
Lafaille JJ et al, J Exp Med 186:307 (1997)

Safety Data Sheet (SDS)

MBP1-17 in TC Media, 100x (PDF)

Related products

EK-0130     MBP1-17/CFA Emulsion