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MBP_1-17 in TC media

For in vitro restimulation of MBP_1-17-specific T cells

Tissue culture tested

In tissue culture media, ready to use

Supplied in vials

For analysis of T cell functions in vitro

Consistently induces in vitro recall responses of T cells isolated from MBP_1-17/CFA-immunized mice

For adoptive transfer EAE in B10.PL, PL/J, or anti-MBP TCR transgenic mice

Induces potent encephalitogenic T cells in vitro

MBP_1-17 in TC media is used in vitro to generate encephalitogenic T cells or to evaluate cellular immune responses.

Encephalitogenic cells are generated by culturing splenocytes from immunized mice for 72 hours in the presence of 20 µg/mL of antigen. To generate encephalitogenic cells, IL-23 or IL-12 and anti-IFNγ antibodies may be added to the culture.

Cat #	Product	Description	Size	SDS (pdf)	Price
DS-0131	MBP_1-17 in TC media	Peptide in tissue culture media	2 mg in 1 mL	SDS	$

Protocol

Cytokine Production Induced by T Cell Recall Response In Vitro

Detailed contents

Myelin basic protein 1-17 (MBP_1-17)
2 mg in 1 mL of tissue culture media (10% fetal bovine serum in RPMI 1640)
Mouse sequence ASQKRPSQRSKYLATAS
Supplied in vials, ready to use

Storage & stability

Stable 3 months (from time of purchase) when stored at -20 °C or one year when stored at -80 °C.
Limit the number of freeze-thaw cycles. Aliquot if using for more than one experiment.