MBP1-17 in TC media
For in vitro restimulation of MBP1-17-specific T cells
- Tissue culture tested
- In tissue culture media, ready to use
Supplied in vials
- For analysis of T cell functions in vitro
Consistently induces in vitro recall responses of T cells isolated from MBP1-17/CFA-immunized mice
- For adoptive transfer EAE in B10.PL, PL/J, or anti-MBP TCR transgenic mice
Induces potent encephalitogenic T cells in vitro
MBP1-17 in TC media is used in vitro to generate encephalitogenic T cells or to evaluate cellular immune responses.
Encephalitogenic cells are generated by culturing splenocytes from immunized mice for 72 hours in the presence of 20 µg/mL of antigen. To generate encephalitogenic cells, IL-23 or IL-12 and anti-IFNγ antibodies may be added to the culture.
| Cat # | Product | Description | Size | SDS (pdf) |
Price |
|---|---|---|---|---|---|
| DS-0131 | MBP1-17 in TC media | Peptide in tissue culture media | 2 mg in 1 mL | SDS | $ 165 |
Protocol
Cytokine Production Induced by T Cell Recall Response In Vitro
Detailed contents
Myelin basic protein 1-17 (MBP1-17)
2 mg in 1 mL of tissue culture media (10% fetal bovine serum in RPMI 1640)
Mouse sequence ASQKRPSQRSKYLATAS
Supplied in vials, ready to use
Storage & stability
Stable 3 months (from time of purchase) when stored at -20 °C
or one year when stored at -80 °C.
Limit the number of freeze-thaw cycles.
Aliquot if using for more than one experiment.
References
Perrin PJ et al, J Immunol 163;1704 (1999)
Sabelko-Downes KA et al, J Exp Med 189:1195 (1999)
Lafaille JJ et al, J Exp Med 186:307 (1997)
Safety Data Sheet (SDS)
MBP1-17 in TC media (PDF)
Related products
EK-0130 MBP1-17/CFA Emulsion
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