News & Blog
2013 May 13 – Hooke Kits™ for EAE induction in Lewis rats available now
Today we're happy to announce the immediate availability of two new Hooke Kits™ for EAE induction in Lewis rats.
Cat. no. EK-3110 (gpMBP69-88/CFA Emulsion) will induce EAE in 9 to 14 week old Lewis rats. EAE develops within 8 to 12 days; all rats fully recover by Day 20 after immunization, without relapses.
Cat. no. EK-3111 (gpMBP69-88/CFA Emulsion PTX) includes pertussis toxin (PTX) in addition to the gpMBP69-88 antigen. This kit will induce EAE in 10 rats; using the PTX, 30% to 60% of rats will experience a single relapse after initial remission. This provides an opportunity for study of mechanism of EAE development and relapses in Lewis rats.
As with our other EAE kits, we have a detailed protocol describing how to use the kits, and matching Hooke Control Kits™ to control for adjuvant-induced responses; these are cat. no. CK-3110, CK-3111, CK-8110, and CK-8111.
2013 May 9 – Updated protocol for EAE Induction by Active Immunization in C57BL/6 Mice
We've updated our recommended protocol for EAE induction in C57BL/6 mice, used with our MOG/CFA peptide emulsion kits with pertussis toxin (cat nos. EK-2110 and EK-2160).
Based on feedback from customers, the new protocol has a simplified description of pertussis toxin (PTX) solution prep, and should be easier to follow and understand.
We do our best, but please contact us if you find any of our protocols unclear - we're always happy to explain and improve things.
2012 October 25 – Improved EAE induction kits with lyophilized PTX
Today we're introducing three improved Hooke Kits™ for EAE induction, all with pertussis toxin (PTX) supplied lyophilized in vials.
The new products have the identical antigen emulsion as our previous products, but come with pertussis toxin as lyophilized powder instead of in PBS.
These new products are the result of our experience with international shipping of EAE induction kits, feedback from customers, and extensive testing, both at Hooke and in customer labs. The potency of PTX in lyophilized form is more stable over variations in transit and storage conditions than when PTX is dissolved in PBS.
The new kits give improved reproducibility of EAE severity, and because lyophilized PTX doesn't lose potency in transit, you'll probably need a lower PTX dose than with the old kits.
As well, because PTX is supplied in powder form, you can adjust dilution to obtain a wide range of PTX concentrations to match conditions in your lab. Therefore each new product replaces several previous products that supplied different amounts of PTX.
We still test each lot of PTX for potency in mice; based on the results, our protocol gives the recommended dilution that will produce consistent EAE, accounting for lot-to-lot variations in PTX potency.
The new products are:
- Hooke Kit™ MOG35-55/CFA Emulsion PTX (cat. no. EK-2110) — for EAE induction in C57BL/6 or (C57BL/6 x SJL)F1 mice
- Hooke Kit™ MOG1-125/CFA Emulsion PTX (cat. no. EK-2160) — for EAE induction in C57BL/6 mice
- Hooke Kit™ PLP139-151/CFA Emulsion PTX (cat. no. EK-2120) — for EAE induction in SJL mice
We've also produced new, much more detailed, recommended protocols for each kit. These include advice on titration for the best PTX dosage, low-stress mouse handling (EAE susceptibility is reduced by stress), a detailed scoring guide, and background biology information.
Although the discontinued products are still available by special order (for customers who don't want to alter existing protocols), we strongly encourage existing customers to switch to these new products. There are no advantages to using the older products.
Along with these improved EAE induction kits, we've also introduced matching Hooke Control Kits™ for customers who need to control for adjuvant-induced responses; these are cat. no. CK-2110, CK-7110, CK-2160, and CK-7160.
Finally, for customers who want to induce more severe EAE, we are offering additional 5 µg vials of lyophilized PTX as a separate item:
This is the same PTX we supply with the new EAE induction kits.
2012 January 11 – Updated protocol for CIA induction
We've updated our recommended protocol for CIA induction in DBA/1 mice, for use with our collagen emulsion products.
The new version of the protocol adds advice on identifying mice likely to develop sub-optimal CIA, and emphasizes the need for very slow delivery of the booster dose (over at least 10 to 15 seconds). Because blood vessels in the tail are dilated as a result of the initial immunization, slow delivery of the emulsion is critical to avoid mouse deaths resulting from formation of embolisms.
2011 June 24 – Frozen tissue from mice with EAE and CIA, frozen CIA serum
We now offer frozen tissue from mice with EAE and CIA (and controls). Like our fresh cell products announced last month, these are a way of "productizing" some of our CRO services, so you can obtain mouse tissue just by ordering a product - without sending your compounds off-site or negotiating an MSA for services.
Before ordering EAE or CIA mouse tissues (doesn't apply to CIA serum), please email us at or for our current inventory - we'll send you an Excel spreadsheet with the details of our frozen inventory (stored at -80 °C), including scoring history for each mouse. You can order individual mice based on disease severity or stage.
All these products ship on dry ice in 1.5 mL cryogenic tubes; we can ship internationally. Most tissue products are always in stock; out-of-stock products are available with a few weeks of lead time.
Snap-frozen spleens, spinal cords, and (optionally) brains from mice with EAE. For analysis of gene expression and protein production.
You can order individual mice based on scoring history, strain (C57BL/6 or SJL), and EAE stage (onset, peak, or recovery for BL/6 mice, or for SJL mice, onset, peak, or remission of the first wave of EAE, or onset, peak, or recovery from relapse).
We also have the same tissues from control mice (no EAE, matched for immunization method and timing) and from naïve mice (no immunization or EAE induction).
One hind paw, half spleen, and serum from a DBA/1 mouse with CIA. For analysis of gene expression, protein production and biomarkers.
Order individual male or female mice from our stock based on scoring history, antigen used for CIA induction (chicken type II collagen or bovine type II collagen) and disease stage (1-2, 4-5, or 14-21 days after CIA onset). We also offer tissues from control mice (no CIA but matched for immunization method and timing) and naïve mice (no immunization or CIA induction).
Frozen serum from DBA/1 mice, taken at 20+ days after onset of CIA induced by immunization with either chicken or bovine type II collagen. For isolation or analysis of anti-collagen antibodies.
Multiple mice are pooled for serum. Serum from control mice (immunized only) and naïve mice (no immunization or CIA induction) is available for comparison.
2011 June 2 – Lower prices on Hooke Emulsion Kits™
The first kit in your order is the same price as before, but all additional kits (of the same catalog number) are discounted by about 20%. That's in addition to our existing 5% and 10% discounts for larger orders.
Because these products have a short shelf life, each Hooke Emulsion Kit™ is made fresh the same day it ships. These discounts reflect our efficiencies from making larger daily batches. (Our lot numbers represent the lot of stable reagents used to produce the fresh emulsion; all kits with the same lot number use the same reagent concentrations and lot.)
2011 May 20 – Fresh cells from immunized mice, MBP1-17 kits, more
We're happy to introduce several new products today. All are available for immediate ordering. As always, contact us at or for more information or to place an order.
First up, we're offering fresh splenocytes and lymph node cells from B6 and SJL mice immunized with MOG35-55 and PLP139-151 peptide antigens (respectively), as well as cells from control mice (immunized with CFA only) and naïve mice (no immunization). These are shipped fresh, overnight, three weeks after your order.
The fresh cells can be used for in vitro analysis of T cell functions including encephalitogenicty, or can be cultured for use in adoptive transfer EAE.
This is a way of "productizing" some of our contract research services - if you don't have your own vivarium, you can order fresh immunized cells as an off-the-shelf product, without spending time negotiating an MSA or sending proprietary compounds out of your facility.
Even if you do have your own mouse lab, our cell quality may be better than your laboratory can produce on an occasional basis, because Hooke routinely immunizes mice and isolates cells using a consistent protocol.
We do the routine work of cell production, so you can spend more time on research.
This new kit induces a strong immune response to MBP1-17 in B10.PL, PL/J, or anti-MBP TCR transgenic mice, for evaluation of MBP1-17-specific proliferation and cytokine production by draining lymph node and spleen cells. Our recommended protocol for this product is Immunization of Mice with Peptide Antigen.
The corresponding Hooke Control Kits™ catalog numbers CK-0130 and CK-5130 are also available.
Last, we've added a MBP1-17 product to our antigens in tissue culture media.
Catalog number DS-0131, MBP1-17 in TC Media, 100x supplies 2 mg of myelin basic protein 1-17 (MBP1-17) peptide in 1 mL of tissue culture media, for in vitro restimulation of MBP1-17-specific T cells.
It's shipped on dry ice, and stable for at least 6 months when stored at or below -20 °C.
Watch this space
We have more new products coming soon - keep an eye on our web page for upcoming announcements of frozen EAE and CIA mouse tissue products, lyophilized whole-MOG antigen, and ELISA kits for detection of collagen-specific and chicken ovalbumin-specific antibodies.
If you need it now, contact us and we'll try to help.
2011 February 3 – Mouse EAE Models presentation
We recently updated our overview presentation covering mouse EAE models and model selection. This discusses the various direct EAE mouse models (C57BL/6 mice immunized with either MOG35-55 peptide or whole MOG protein, SJL mice immunized with PLP139-151), adoptive transfer EAE models, possible treatment regimens (prophylactic, semi-therapeutic, therapeutic, late therapeutic), controls, typical results, etc. It also provides some guidance on selection of the best model, depending on your goals and how much you already know about your compound and its target.
This is an updated version of a PowerPoint presentation we've offered to CRO customers for some time. Kit customers working with EAE models may also find it useful.
2010 December 13 – Sample IACUC protocol available
We've just posted a sample IACUC protocol for EAE in C57BL/6 mice. We hope our customers will find this useful as a starting point for drafting their own IACUC protocols. We're working on additional sample IACUC protocols, for different models. These will be available with the other protocols on our protocols page.
You are welcome to download, use, and modify these protocols, free of charge, in your own institution. We'd appreciate credit to Hooke as a courtesy, but it's not required. Suggestions for other useful protocols, or for improvements, are always welcome - just email us at .
2010 November 29 – Pertussis toxin recommendations for MOG EAE induction kits
We've changed our recommendation for new users of our MOG EAE induction kits; based on customer feedback we are now suggesting that new users start with EK-0114 with 3.75x pertussis toxin (PTX) instead of EK-0115 with 5x PTX. Existing customers who are using EK-0115 might want to try switching to EK-0114; it may work just as well for you, and it's a little cheaper.
We offer MOG EAE induction kits with PTX concentrations ranging from 1x (EK-0113) to 5x (EK-0115) because the amount of PTX needed to produce good EAE varies, quite a lot, from lab to lab. Many factors affect the amount of PTX needed, but the one you have the most control over is stress - stress reduces EAE susceptibility in mice, so low-stress mouse handling procedures reduce the amount of PTX needed. We see that customers who do all procedures in the mouse room (instead of moving mice around on carts) usually need less PTX to induce EAE.
Of course stress is not the only factor involved. Our recommendation is to start with EK-0114 and then move to a weaker or stronger kit if necessary, depending on the results you get. In our own CRO lab, we find EK-0110 with 1.5x PTX produces the best disease, but most of our customers find PTX concentrations in the range of 2.5x (EK-0112) to 3.75x (EK-0114) works best for them.
Customers sometimes ask why we describe the PTX concentration in "x" units instead of mg/mL. We want our kits to consistently have the same strength and produce consistent EAE, but the potency of the reagents we buy varies considerably from lot to lot. So we characterize each lot in live mice and adjust the concentration accordingly. Our "5x" kit will always have five times as much PTX as our "1x" kit, but the actual concentration will vary depending on the potency of PTX used. The lot number on each kit represents which lot of reagents was used to produce the kit; each kit is made fresh on the day we ship it, but all kits with the same lot number will have the same concentration of each reagent.
In addition to reagent quantities, the quality of the emulsion is critical to EAE induction. Our recommended protocol for these kits has many more tips and suggestions for reliable EAE induction.
2010 September 29 – No more packing fees, new "News & Blog" section
Effective today, we're no longer charging a $25 "packing fee" on product orders.
This fee was meant to cover the cost of the packaging, dry ice, and gelpacks we use to ship products, but we've decided to drop the fee and "eat" these costs - nobody likes to pay more than they expect on their order, and shipping costs for the large insulated packages are already higher than we'd like. The fixed per-order packing fee had a big impact on the total cost of small orders; we hope you'll feel more comfortable placing small orders.
Today's other change is the evolution of our old "News & Press" section on Hooke's web site to the new "News & Blog" section you're reading now. In addition to product announcement/press release information, we plan to use this for more informal, and more frequent, communication with our customers. We hope you'll find it useful.
2010 April 16 – Three new IFA Emulsion Hooke Kits™: Ovalbumin, Pigeon Cytochrome c, and Hen Egg Lysozyme/IFA emulsions
Hooke Laboratories is pleased to announce the immediate availability of three new Hooke Emulsion Kits™ and their associated Hooke Control Kits™:
- Ovalbumin/IFA Emulsion, catalog number EK-0311
- Pigeon Cytochrome c/IFA Emulsion, catalog number EK-0312
- Hen Egg Lysozyme/IFA Emulsion, catalog number EK-0313
Each new Hooke Emulsion Kit™ contains antigen emulsified in Incomplete Freund's Adjuvant (IFA).
These Hooke Kits™ will induce production of high concentrations of antibodies specific to each antigen. A detailed protocol describing use of these kits is available on Hooke's web site.
Each kit is delivered in pre-filled syringes, ready to use, and contains enough emulsion for immunization of 20 mice.
2010 April 12 – Updated protocol for EAE induction by active immunization in SJL mice
Hooke Laboratories, Inc. has issued an updated version of their recommended protocol for EAE Induction by Active Immunization in SJL Mice, for use with Hooke Emulsion Kits™ PLP139-151/CFA Emulsion (EK-0120), PLP139-151/CFA Emulsion PTX (EK-0121), and PLP139-151/CFA Emulsion PTXx2 (EK-0123).
The updated protocol recommends 4 injections of 0.05 mL per mouse, instead of 2 injections of 0.1 mL per mouse. This modified protocol induces EAE in a larger proportion of mice (at least 80% of mice), with less variability from experiment to experiment.
The revised protocol is available for immediate download from Hooke's web site.
2009 August 24 – Pigeon Cytochrome c, Hen Egg Lysozyme Immunization Kits
These two new immunization kits expand Hooke's range of kits for inducing immune response in rodents.
Also introduced today are a set of accompanying Hooke Control Kits™: CK-0302, CK-5302, CK-0303, and CK-5303. These are identical to the newly introduced Hooke Emulsion Kits™, except that they contain no specific antigens, and may be used to control for effects of the adjuvant components in Hooke Emulsion Kits™.
All the products introduced today are available immediately.
2009 August 18 – MOG1-125 Hooke Emulsion Kits™ for EAE induction
Hooke Laboratories, Inc. is pleased to announce the immediate availability of two new Hooke Emulsion Kits™ for induction of EAE in C57BL/6 mice, catalog numbers EK-0162 and EK-0164 – MOG1-125/CFA Emulsion PTX, as well as a set of associated Hooke Control Kits™.
These products are recommended for testing therapeutics aimed at impairing B-cell function, as they induce EAE using human recombinant myelin oligodendrocyte glycoprotein (1-125) (MOG1-125).
Published clinical data has shown that B-cells play a pathogenic role in human multiple sclerosis (MS).
Unlike peptide-induced EAE (using antigens MOG35-55 or MBP1-11), human MOG1-125 induced EAE is reported to be dependent on B-cell presence. Therefore MOG1-125-induced EAE is likely a good model for testing therapeutics which target B-cells.
2009 August 10 – New "PTXx2" PLP139-151/CFA Emulsion, updated protocol
Hooke Laboratories, Inc. has introduced a new Hooke Emulsion Kit™, PLP139-151/CFA Emulsion PTXx2 (EK-0123) which provides for a second pertussis toxin (PTX) injection. The new kit joins Hooke's existing EK-0120 and EK-0121 products intended for induction of EAE in SJL mice.
The new EK-0123 Hooke Kit™ is recommended for observation of incidence and severity of the first wave of EAE, as it induces EAE with higher incidence and greater severity in the first wave.
Hooke's protocol for EAE induction in SJL mice has also been updated, both to provide guidance on the use of the new Hooke Kit™, and to provide detailed illustrations of procedures for successful EAE induction.
2009 May 15 – Family of Hooke Control Kits™ Released
A complete line of Hooke Control Kits™ are now available to accompany Hooke Emulsion Kits™.
Hooke Control Kits™ enable distinction between responses induced by adjuvant components of Hooke Emulsion Kits™ and those induced by a specific antigen. This distinction is important in the study of immune response mechanisms. Hooke Control Kits™ are identical to the corresponding Hooke Emulsion Kits™, except that they contain no specific antigens.
Most kits are offered in two sizes – a full size kit with the same quantity of emulsion provided in the corresponding Hooke Emulsion Kit™, and a half size kit suitable for smaller control groups.
The adjuvant components of Hooke Emulsion Kits™ typically induce immune responses including skin inflammation, enlargement of spleen, and increase in cytokine production. These effects result from the adjuvant component of the kit, not the specific antigen component. Like Hooke Emulsion Kits™, the new products are supplied in pre-filled syringes, ready to use.
Detailed information on the new products is available online at http://hookelabs.com.
2009 January 26 – Bovine Type II Collagen Emulsion Hooke Kits™
Hooke Laboratories, Inc. has announced the immediate availability of two new Hooke Kit™ products for induction of collagen-induced arthritis (CIA), one of the most commonly used animal models of human rheumatoid arthritis, in DBA/1 mice.
Hooke Kit™ EK-0220, Bovine Collagen/CFA Emulsion, is used to immunize DBA/1 mice with bovine type II collagen emulsified in complete Freund's adjuvant (CFA). Three weeks later, a booster dose of bovine type II collagen in incomplete Freund's adjuvant (IFA) is administered using Hooke Kit™ EK-0221, Bovine Collagen/IFA Emulsion.
CIA is consistently induced by these new Hooke Kits™ 16-35 days after immunization in 90–100% of male DBA/1 mice, and in 60–100% of female DBA/1 mice.
The pre-characterized Hooke Kits™ introduced today are supplied in pre-filled syringes, ready to use. Each lot is extensively tested and adjusted for consistent potency and reliable CIA induction.
Full product details and recommended experimental protocols are available on the company's web site, http://hookelabs.com.
2009 January 9 – Updated EAE Induction Protocol
Hooke Laboratories has released an updated version of the EAE Induction by Active Immunization in C57BL/6 Mice protocol for use with MOG35-55/CFA Emulsion PTX Hooke Kits™.
This updated protocol provides more detailed instructions with illustrations of procedures for successful EAE induction.
Successful EAE induction requires low-stress mouse handling and husbandry procedures, good injection technique (see detailed illustrations in the protocol), use of appropriate mice, and good quality, stable, antigen emulsion together with the correct amount of pertussis toxin.
For reliable EAE induction, the protocol recommends:
- Mice should be acclimated for 7 days to your lab before starting.
- Use female mice at no younger than 9 weeks old. (10 weeks or older recommended.)
- Follow recommended injection procedure (see illustrations in protocol).
- Avoid excessive noise or vibration.
- Avoid excessive handling of mice. Handle mice gently.
- Try to do all procedures in the mouse room. Avoid moving mice on carts.
- Static cages are preferred to (some) ventilated cages.
- Avoid use of anesthesia if possible. If anesthesia must be used, we recommend inhaled anesthesia.
The updated protocol is available immediately on Hooke's web site.
2008 December 15 – MOG35-55 and PLP139-151 Peptides in Solution
Hooke Laboratories is pleased to announce the immediate availability of two new peptide products, MOG35-55 in TC Media, 100x (catalog number DS-0111), and PLP139-151 in TC Media, 100x (catalog number DS-0121).
These peptides are offered in tissue culture media (10% fetal bovine serum in RPMI 1640), ready to use.
They are suitable for analysis of T cell functions in vitro, and for production of encephalitogenic T cells in vitro for use in adoptive transfer models of experimental autoimmune encephalomyelitis (EAE).
2008 November 24 – PLP139-151/CFA Emulsion PTX Hooke Kit™
This Hooke Kit™ is intended for induction of remitting/relapsing EAE in female SJL mice.
The first wave of EAE will develop 10 to 14 days after immunization in 90 to 100% of mice, with a mean maximum severity of approximately 3.5 (scale 0–5).
Relapse will start around 25 days after immunization. By 40 days after immunization, approximately 50% of mice will relapse. Mean maximum severity of relapses will be around 2 (scale 0–5).
Two 1 mL syringes of PLP139-151/CFA emulsion and one 1 mL syringe of pertussis toxin are included, sufficient for 10 mice. A detailed protocol and MSDS data are available online.
2008 October 6 – New Ovalbumin/CFA Emulsion Hooke Kit™
This new Hooke Kit™ can be used to immunize mice (and other species) for evaluation of cellular immunity and antibody responses, as well as induction of delayed-type hypersensitivity (DTH).
As with all Hooke Kits™, Ovalbumin/CFA Emulsion is delivered in pre-filled syringes, ready to use. Each EK-0301 kit contains enough emulsion for immunization of 5 to 20 mice, depending on dose.
2008 September 29 – Stronger MOG35-55/CFA emulsion Hooke Kits™
These kits are available immediately and contain MOG35-55 peptide emulsified in Complete Freund's Adjuvant (CFA), with larger amounts of pertussis toxin (PTX) compared to our existing MOG35-55/CFA Emulsion PTX Hooke Kits™.
With these new products, Hooke offers a PTX dosage range of 1x to 5x, to accommodate a wider variety of mouse age, strain, breeder, and colony. Hooke Kits™ with higher doses of PTX will induce EAE of greater severity.
We recommend that new customers run 10 mice with our strongest Hooke Kit™ (EK-0115) and let us recommend the best Hooke Kit™ based on the results (EAE incidence and average severity).
Pertussis toxin enhances EAE development by providing additional adjuvant, compared to CFA alone. Injection of PTX affects cell trafficking, development of Th17 cells and integrity of the blood-brain barrier. In our experience, successful induction of EAE requires both good quality antigen emulsion and the appropriate amount of pertussis toxin.
Kits Accelerate Science by Saving Time In Testing, Ensuring Successful In Vivo Experiments
Hooke Laboratories, Inc. today announced the introduction of seven Hooke Kit™ products for immunization and induction of EAE and CIA in mice. For the first time, scientists studying autoimmune diseases such as arthritis and multiple sclerosis will no longer have to tediously prepare emulsions and test individual reagents before starting experiments.
The pre-characterized Hooke Kits™ introduced today are supplied in pre-filled syringes, ready to use. They ensure reliable and consistent disease induction, as each lot is extensively tested and individually adjusted for use with different sub-strains and ages of laboratory mice.
Properly prepared emulsions are critical for reliable induction of many autoimmune disease models, such as experimental autoimmune encephalomyelitis (EAE, a mouse model of human multiple sclerosis) and collagen-induced arthritis (CIA, one of the most commonly used animal models of human rheumatoid arthritis). Hooke's emulsions are pre-made and pre-tested to reduce the time needed to set up experiments.
Suzana Marusic, MD, PhD, President and Founder of Hooke Laboratories said "We're really delighted with the consistent EAE and CIA induction rates we've been able to achieve with these kits. By choosing a Hooke Kit™ with potency adjusted for particular mouse sub-strains and ages, our customers can induce CIA in 100% of DBA/1 mice of either Taconic or Jackson origin – even in females, which is usually extremely difficult."
The seven Hooke Kit™ products announced today are:
Hooke Kits™ MOG35-55/CFA Emulsion PTX for EAE Induction
- MOG35-55/CFA Emulsion PTX – catalog numbers EK-0110, EK-0112, EK-0113
Hooke Kits™ for Immunization
Hooke Kits™ for CIA Induction
- Chicken Collagen/CFA Emulsion – catalog number EK-0210
- Chicken Collagen/IFA Emulsion – catalog number EK-0211
Additional Hooke Kit™ products are in development, including Bovine Type II collagen emulsions for induction of CIA.
Full product details, including recommended experimental protocols and typical result data, are available on the company's web site, http://hookelabs.com.
Hooke Laboratories offers innovations that make animal research more efficient and less expensive, with the goal of accelerating cures for human diseases while reducing and refining the use of animals. Hooke is working to be the leading company providing tools for in vivo research. The company was founded by immunologist Suzana Marusic, MD, PhD.