Frozen tissue from mice with EAE
For analysis of gene expression and protein production
Snap-frozen mouse tissue from well-defined stages and severity of EAE, and control tissues. Choose tissue samples from our inventory based on EAE severity, disease stage, or mouse strain. Shipped frozen on dry ice.
- Analyze gene expression and protein presence in EAE tissue without a vivarium
Save time establishing & running EAE models; most tissue products are available from stock with no lead time.
- Choose individual mice from our inventory based on EAE severity
Full EAE score history provided for each sample.
- Tissue from control and naïve mice available for comparison
Control tissues are matched for immunization method and timing.
Availability (IMPORTANT)
Most tissue products are available from stock with no lead time, but contact us at or for a complete listing of our current inventory in Excel format, including EAE score history for each mouse.
You can choose individual mice from our stock based on EAE severity, disease stage, or mouse strain.
Note: Brain tissue is also available for some mice (add $12/mouse). See our current inventory for availability.
EAE tissue
| Cat # | Strain | Disease stage | Tissue | Size | Price |
|---|---|---|---|---|---|
| EZ-1110 | C57BL/6 | EAE onset | Spleen & spinal cord | 1 mouse | $ 205 |
| EZ-1120 | C57BL/6 | EAE disease peak | Spleen & spinal cord | 1 mouse | $ 205 |
| EZ-1130 | C57BL/6 | EAE recovery (chronic phase) | Spleen & spinal cord | 1 mouse | $ 205 |
| EZ-1210 | SJL | Onset of first wave of EAE | Spleen & spinal cord | 1 mouse | $ 219 |
| EZ-1220 | SJL | Peak of first wave of EAE | Spleen & spinal cord | 1 mouse | $ 219 |
| EZ-1230 | SJL | Remission from first wave of EAE | Spleen & spinal cord | 1 mouse | $ 219 |
| EZ-1240 | SJL | Onset of EAE relapse (within 24 hours after onset) | Spleen & spinal cord | 1 mouse | $ 438 |
| EZ-1250 | SJL | Peak of EAE relapse (2-3 days after onset) | Spleen & spinal cord | 1 mouse | $ 438 |
| EZ-1260 | SJL | Recovery from EAE relapse | Spleen & spinal cord | 1 mouse | $ 438 |
Control tissue
Control tissue from mice immunized with control kit (no EAE induction), matched for time from immunization.
| Cat # | Strain | Disease stage | Tissue | Size | Price |
|---|---|---|---|---|---|
| EZ-1111 | C57BL/6 | Immunized only, time matched to EAE onset | Spleen & spinal cord | 1 mouse | $ 205 |
| EZ-1121 | C57BL/6 | Immunized only, time matched to EAE peak | Spleen & spinal cord | 1 mouse | $ 205 |
| EZ-1131 | C57BL/6 | Immunized only, time matched to EAE recovery | Spleen & spinal cord | 1 mouse | $ 205 |
| EZ-1211 | SJL | Immunized only, time matched to EAE first wave onset | Spleen & spinal cord | 1 mouse | $ 219 |
| EZ-1221 | SJL | Immunized only, time matched to EAE first wave peak | Spleen & spinal cord | 1 mouse | $ 219 |
| EZ-1231 | SJL | Immunized only, time matched to EAE first wave remission | Spleen & spinal cord | 1 mouse | $ 219 |
| EZ-1241 | SJL | Immunized only, time matched to EAE relapse onset | Spleen & spinal cord | 1 mouse | $ 219 |
| EZ-1251 | SJL | Immunized only, time matched to EAE relapse peak | Spleen & spinal cord | 1 mouse | $ 219 |
| EZ-1261 | SJL | Immunized only, time matched to EAE relapse recovery | Spleen & spinal cord | 1 mouse | $ 219 |
Naïve tissue
Tissue from naïve mice (no immunization or EAE induction). For control purposes.
| Cat # | Strain | Disease stage | Tissue | Size | Price |
|---|---|---|---|---|---|
| EZ-1100 | C57BL/6 | Naïve mouse (no immunization or EAE induction) | Spleen & spinal cord | 1 mouse | $ 75 |
| EZ-1200 | SJL | Naïve mouse (no immunization or EAE induction) | Spleen & spinal cord | 1 mouse | $ 89 |
Tissue preparation
Female C57BL/6 or SJL mice 8 to 12 weeks of age are acclimated to our facility for at least 7 days.
In C57BL/6 mice, EAE is induced according to the protocol EAE Induction by Active Immunization in C57BL/6 Mice.
In SJL mice, EAE is induced according to the protocol EAE Induction by Active Immunization in SJL Mice.
Mice are scored daily for EAE severity on the scale of 0-5 in 0.5 unit increments (see protocol).
Mice are weighed 3 times/week (Monday, Wednesday, Friday).
Mice are euthanized at the appropriate stage of disease. Mice are immediately perfused with PBS, then spleen & spinal cord are isolated and snap frozen in liquid nitrogen.
Tissues are stored at -80 C until shipment.
Detailed contents
Each product unit contains the entire spleen and spinal cord tissue from a single mouse.
Each organ is supplied in a separate 1.5 mL cryogenic tube. Tubes are marked with a mouse ID and tissue weight. (Typical spinal cords are 80 to 100 mg each, spleens are typically 100 to 200 mg.)
The daily EAE severity scores and weight history for the mouse are provided for each mouse as an Excel sheet, via email. (Normally customer chooses mice for ordering based on EAE score history.)
Storage
Keep frozen until use. Storage at -80 C is recommended.
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